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ProteomicsBait correlation improves interactor identification by TMT-AP-MS
The quantitative multiplexing capacity of isobaric Tandem Mass Tags (TMT) has increased the throughput of affinity purification mass spectrometry (AP-MS) to characterize protein interaction networks of immunoprecipitated baits. However, variable bait levels between replicates can convolute interacto...
ORGANISM(S): Homo sapiens (Human) 
2020-06-22 | PXD016613 | Pride
Tmt Protein-protein interactions Ap-ms 
UnknownGlobal quantitative proteomic analysis profiles of host protein expression in response to enterovirus A71 infection in bronchial epithelial cells based on tandem mass tag (TMT) peptide labeling coupled with LC-MS/MS uncovers the key role of proteasome in virus replication.
Not available
S-EPMC10194169 | biostudies-literature
UnknownComparative Proteome Profiling of Saliva Between Estrus and Non-Estrus Stages by Employing Label-Free Quantitation (LFQ) and Tandem Mass Tag (TMT)-LC-MS/MS Analysis: An Approach for Estrus Biomarker Identification in Bubalus bubalis.
Not available
S-EPMC9217162 | biostudies-literature
ProteomicsMultiple sclerosis biomarker discovery in cerebrospinal fluid using glycopeptide enrichment, TMT labeling and LC-MS/MS
Multiple sclerosis biomarker discovery in pooled cerebrospinal fluid (CSF) using glycopeptide enrichment, TMT labeling and LC-MS/MS. Comparing 3 pools of CSF from relapsing-remitting MS (RRMS) patients to 3 pools of CSF from patients with other neurological diseases (OND). To reveal protein groups a...
ORGANISM(S): Homo sapiens (Human) 
2016-10-13 | PXD004540 | Pride
Human Cerebrospinal fluid Tmt Discovery Glycopeptide enrichment Lc-ms/ms 
ProteomicsOne-STAGE Tip Method for TMT based Proteomic Analysis of Minimal Amount of Cells
LC-MS-based profiling of proteomes with isobaric tag labeling from low-quantity biological and clinical samples, including needle-core biopsies and laser capture microdissection, has been challenging due to limited amount and sample loss during preparation. To address this problem, we developed OnM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-18 | MSV000091734 | MassIVE
UnknownIdentification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.
Not available
S-EPMC7396582 | biostudies-literature
ProteomicsPseudomonas aeruginosa PAO1 TMT proteomics analysis RPLC-MS
The studied compound SN12 significantly inhibited biofilm formation in Pseudomonas aeruginosa, but its regulatory mechanism is unknown. To determine the mechanism of action, TMT tagging was used to analyse the proteomics of P. aeruginosa PAO1. Three groups of blank controls treated with PBS were com...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2025-05-06 | PXD047035 | Pride
Proteomics Tmt Rplc-ms Pseudomonas aeruginosa 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsEimeria necatrix Wall-Forming Bodies and Oocyst Wall Proteomic Analysis by Tandem Mass Tag (TMT) Labeling and Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) Techniques
WFBs and the oocyst wall of Eimeria necatrix were subjected to comparative proteomic analysis using tandem mass tag (TMT) in conjunction with liquid chromatography tandem-mass spectrometry (LC-MS/MS) techniques
ORGANISM(S): Eimeria necatrix 
2024-01-26 | PXD042839 | Pride
Wall-forming bodies Tmt Eimeria necatrix Oocyst wall Lc-ms/ms Comparative proteomics 
ProteomicsTMT combined with LC–MS/MS technique to detect protein markers in gastroesophageal junction adenocarcinoma
Gastroesophageal junction adenocarcinoma (GEJA) is a malignant tumor located at the junction of the esophagus and stomach, and its incidence is increasing yearly, while early screening for biological markers is limited. Tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectromet...
ORGANISM(S): Homo Sapiens 
2021-12-08 | PXD030228 |
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