In order to investigate the effect of the NLRP3 inflammasome on the regeneration of enthesis, we established an enthesis injury and repair model in wild-type and NLRP3-/- mice. The interstitial fluid of enthesis was extracted, and untargeted metabolomics was conducted to discover differential metabo...
Untargeted metabolites of supernatant of wild-type and Nlrp3-/- bone marrow-derived macrophages without stimulation or with 4 hours of LPS preconditioning and subsequently 30 min of ATP stimulation.
Lei et al. screen out Lactococcus lactis OMVs (Lac-OMVs) and develop metabolic-engineered NAD+-Lac-OMVs nanovaccine with potent tissue repair therapeutic effect. NAD+-Lac-OMVs sequentially activate dendritic cells by membrane component and reprogram inflammatory macrophages for anti-inflammation and...
Low back pain (LBP), often caused by intervertebral disc degeneration (IVDD), poses a major challenge in aging populations. DDX1, an RNA-binding protein, plays key roles in RNA metabolism but its function in IVDD remains unclear. We identify DDX1 as a non-histone substrate of lysine methyltransferas...
To identify the proteins within the EVs that play major roles in mediating EV sorting into lysosomes, we used a biotin-labelled method to monitor the proteomic change associated with EVs during their degradation.
