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TranscriptomicsTop1 and Top2-mediated replication fork integrity
DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chroma...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-05 | E-GEOD-8305 | biostudies-arrayexpress
TranscriptomicsLigand-Dependent Enhancer Activation Regulated by Topoisomerase-I Activity
The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stress f...
ORGANISM(S): Homo sapiens 
2015-01-22 | E-GEOD-63202 | biostudies-arrayexpress
Transcriptomicstop1
Thebaine and oripavine accumulating mutant (Norman) verses wildtype. Keywords = Opium Poppy, Secondary Metabolism
ORGANISM(S): Papaver somniferum 
2010-06-11 | E-GEOD-1543 | biostudies-arrayexpress
ProteomicsTEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts
Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. TOP1 is also a major driver of endogenous genome instability, particularly when its catalytic intermediate - a covalent TOP1-DNA adduct known as a TOP1 cleavage complex (TOP1cc) - is stabi...
ORGANISM(S): Homo sapiens (Human) 
2020-03-13 | PXD017239 | Pride
Protein-dna adducts Vcp/p97 Top1 Tex264 Dna repair Genome stability Sprtn Dna replication 
TranscriptomicsTranscription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin
To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-MEXP-1104 | biostudies-arrayexpress
TranscriptomicssiRNA profiling of stable HCT116-siTop1 and MCF-7-siTop1 cell lines reveal non-classical functions of human Topoisomerase I:
The biological functions of nuclear topoisomerase I (Top1) have been difficult to study because knocking out TOP1 is lethal in metazoans. To reveal the functions of human Top1, we have generated stable Top1siRNA cell lines from colon and breast carcinomas (HCT116-siTop1 and MCF-7-siTop1, respectivel...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7161 | biostudies-arrayexpress
TranscriptomicsRNA-seq of LPS-challenged microglial cell treatment with TOP1 inhibitor
This dataset contains the RNA-seq data generated to investigate the mechanism of TOP1 inhibitor topotecan (TPT)'s role of anti-inflammation in microglial. RNA from mouse microgial cells, cells with LPS treatment to induce inflammation, and cells with LPS and TPT treatment were harvest and processed ...
ORGANISM(S): Mus musculus 
2022-04-27 | E-MTAB-10679 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of novel splice variants induced by topoisomerase I poisoning shows preferential occurrence in genes encoding splicing factors
Topoisomerase I (Top1) relaxes both positive and negative supercoilings by producing transient Top1 cleavage complexes (Top1cc). Several studies have suggested the implication of Top1 in splicing. Here, we tested the implication of Top1cc in splicing at the global genome level in human carcinoma cel...
ORGANISM(S): Homo sapiens 
2011-07-21 | E-GEOD-23677 | biostudies-arrayexpress
ProteomicsThe plant thimet oligopeptidases TOP1 and TOP2 have divergent redox sensitivities and substrate cleavage specificity
Physiological and pathogen-induced oscillations in the plant cells' redox environment trigger response mechanisms that involve redox-sensitive cysteines and consequent changes in proteins' biochemical and functional characteristics. Arabidopsis thimet oligopeptidases (TOPs) TOP1 and TOP2 are compone...
ORGANISM(S): Escherichia coli 
2021-04-19 | PXD024059 | Pride
Arabidopsis Redox regulation Thimet oligopeptidase 
GenomicsSingle cell RNA sequencing of P7 cortex from Top1 knockout mice
Single cell RNA sequencing of P7 cortex from Top1 knockout mice
PRJNA611603 | ENA
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