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TranscriptomicsRNA-sequencing of the human milk fat layer during colostrum, transitional, and mature stages of lactation
Human milk fat globules, by enveloping cell contents during their secretion into milk, are a rich source of mammary cell RNA. Here, we pair this non-invasive mRNA source with RNA sequencing technology to probe the milk fat layer transcriptome during three stages of lactation: colostral, transitional...
ORGANISM(S): Homo sapiens 
2013-07-22 | E-GEOD-45669 | biostudies-arrayexpress
MetabolomicsHuman milk as infantile Super Food - the effect of dietary choices and supplementation on the shift of lipidomic profile during different stages of lactation
The objective of this project was to establish an advanced analytical method leveraging high-resolution mass spectrometry LC-HRMS to comprehensively profile the human milk lipidome. Mass spectrometry resolves and quantifies compounds based on a fundamental physical metric: the mass-to-charge ratio. ...
2026-09-22 | MTBLS15678 | MetaboLights
MetabolomicsMulti-omics and intervention analyses reveal a hindgut microbiota–secondary bile acid–mammary axis linked to milk yield in dairy cows
Background In large scale dairy farming systems, maintaining stable milk yield post-lactation peak is crucial for dairy farms’ economic benefits. While sufficient evidence shows the microbiota profoundly impacts host production efficiency, the specific mechanisms by which hindgut microbiota support ...
2026-05-22 | MTBLS14549 | MetaboLights
MetabolomicsMulti-omics and intervention analyses reveal a hindgut microbiota–secondary bile acid–mammary axis linked to milk yield in dairy cows_Mus musculus_Serum

Background

In large scale dairy farming systems, maintaining stable milk yield post-lactation peak is crucial for dairy farms’ economic benefits. While sufficient evidence shows the microbiota profoundly impacts host production efficiency, the specific mechanisms by which hindgut microbiota...

2026-04-15 | MTBLS14063 | MetaboLights
MetabolomicsMulti-omics and intervention analyses reveal a hindgut microbiota–secondary bile acid–mammary axis linked to milk yield in dairy cows_serum

Shotgun metagenomics, untargeted metabolomics, and targeted metabolomics of high versus normal-yield cows revealed that high-yield animals harbored distinct hindgut microbial communities enriched in bile salt hydrolase–positive taxa, and elevated levels of deoxycholic acid (DCA). Fecal microbiota...

2026-04-15 | MTBLS14061 | MetaboLights
ProteomicsGOAT DAIRY PROTEOMICS AND VALORIZATION
This study aimed to characterize the whey proteome of colostrum, transitional milk, and mature milk from Murciano-Granadina goats, and to evaluate the use of transitional milk as a substitute for colostrum in feeding neonatal kids. Proteomic analysis identified a total of 1,385 proteins, with 736 in...
ORGANISM(S): Capra hircus (Goat) 
2026-04-29 | PXD068261 | Pride
ProteomicsBovine colostrum, transitional and mature milk alpha-lactalbumin N-glycosylation
α-Lactalbumin is an abundant protein present in the milk of most mammals, and is associated with biological, nutritional and technological functionality. Its sequence presents N-glycosylation motifs, the occupancy of which is species-specific, ranging from no to full occupancy. Here, we investigated...
ORGANISM(S): Bos taurus (Bovine) 
2024-08-09 | PXD050331 | Pride
Glycosylation Bovine colostrum Alpha-lactalbumin Bovine milk 
ProteomicsCharacterization and functional differentiation of colostrum, transition milk, and mature milk somatic cells proteomes in multiparous Murrah buffaloes
The objective of the present study was to examine the proteome dynamics of somatic cells from colostrum to mature milk. Milk somatic cells are a mixture of mammary epithelial cells (MECs) and immune cells, secreted in milk during milking. Besides protecting the mammary gland, the milk somatic cells ...
ORGANISM(S): Bos taurus (Bovine) 
2025-07-14 | PXD054309 | Pride
Milk somatic cells Proteomics Transitional milk Murrah buffaloes Colostrum Mature milk Lc-ms/ms 
TranscriptomicsRNA-Seq data from the milk somatic cell transcriptome of primiparus Assaf dairy sheep
This research investigates the influence of nutritional protein restriction (NPR) during prepuberty on FE and the milk transcriptome of dairy Assaf ewes during their first lactation. Additionally, it evaluates the differences in the milk transcriptome between lactating ewes with divergent FE using t...
ORGANISM(S): Ovis aries 
2024-03-01 | E-MTAB-13619 | biostudies-arrayexpress
ProteomicsShotgun proteome analysis of homogenate milk samples reveals dynamic changes in milk proteins between colostrum, transitional and mature milk of swine
Milk is an easily digestible source of nutrients and bioactive factors, and its composition reflects the neonate's needs and changes from colostrum to transitional and mature milk. Our objective was to measure milk fat, lactose, total carbohydrate, and protein content in parallel with global proteo...
ORGANISM(S): Sus Scrofa (ncbitaxon:9823) 
2021-03-25 | MSV000087094 | MassIVE
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