Sort   by:  
 Page size 
TranscriptomicsThe ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
Recent studies highlight the importance of translational control in determining protein abundance, underscoring the value of measuring gene expression at the level of translation. We present a protocol for genome-wide, quantitative analysis of in vivo translation by deep sequencing. This ribosome pr...
ORGANISM(S): Homo sapiens 
2012-07-26 | E-GEOD-37744 | biostudies-arrayexpress
ProteomicsSuppressed Protein Translation Caused by MSP-8 Defect Determines Fungal Multidrug Resistance with Fitness Cost
Antifungal resistance, particularly the rise in multidrug resistance, has become a critical public health concern, garnering substantial attention. In this study, we used experimental evolution with Neurospora crassa as a model and identified a novel multidrug-resistance gene, msp-8, which encodes a...
ORGANISM(S): Neurospora Crassa Or74a 
2024-08-02 | PXD054574 |
TranscriptomicsGenome-Wide Analysis In Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling
Techniques for systematically monitoring protein translation have lagged far behind methods for measuring mRNA levels. Here we present a ribosome profiling strategy, based on deep sequencing of ribosome protected mRNA fragments, that enables genome-wide investigation of translation with sub-codon re...
ORGANISM(S): Saccharomyces cerevisiae 
2009-02-12 | E-GEOD-13750 | biostudies-arrayexpress
ProteomicsSystemic remodeling of translation efficiencies on oxygen stimulus.
Protein concentrations evolve under greater evolutionary constraint than mRNA levels. In addition, translation efficiency of mRNA populations represents the chief determinant of basal protein concentrations. This raises a fundamental question as to how mRNA and protein levels are actively coordina...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-20 | MSV000079962 | MassIVE
TranscriptomicsImproved transcription and translation with L-leucine stimulation of mTORC1
Roberts syndrome (RBS) is a human developmental disorder caused by mutations in the cohesin acetyltransferase ESCO2. We previously reported that mTORC1 was inhibited and overall translation was reduced in RBS cells. Treatment of RBS cells with L-leucine partially rescued mTOR function and protein sy...
ORGANISM(S): Homo sapiens 
2016-01-15 | E-GEOD-64962 | biostudies-arrayexpress
Transcriptomicsm6A in 5’ untranslated regions promotes cap-independent translation of mRNA
N6-methyladenosine (m6A) is a widespread internal RNA modification whose function is poorly understood. Here we report that m6A residues within the 5'UTR promote a novel form of cap-independent translation which is mediated through an interaction between m6A residues and the translation initiation f...
ORGANISM(S): Homo sapiens 
2015-10-26 | E-GEOD-73405 | biostudies-arrayexpress
TranscriptomicsDistinct stages of the translation elongation cycle revealed by sequencing ribosome-protected mRNA fragments
During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-10 | E-GEOD-58321 | biostudies-arrayexpress
TranscriptomicsA unifying model for mTORC1-mediated regulation of mRNA translation
Ribsome profiling analysis of mRNA translation in mouse cells under conditions of mTOR activiation or inhibition. embryonic fibroblasts from 4EBP1/2 p53 mutants treated with Torin1
ORGANISM(S): Mus musculus 
2012-05-02 | E-GEOD-36892 | biostudies-arrayexpress
TranscriptomicsRibosome profiling data obtained from HEK293T cells 30 minutes after treatment with arsenite to a final concentration of 40 M-BM-5M
Eukaryotic cells rapidly reduce protein synthesis in response to various stress conditions. This can be achieved by the phosphorylation-mediated inactivation of a key translation initiation factor, eIF2. However, the persistent translation of certain mRNAs is required for deployment of an adequate s...
ORGANISM(S): Homo sapiens 
2015-01-27 | E-GEOD-55195 | biostudies-arrayexpress
TranscriptomicsRibosome profiling with translation inhibitors reveals pervasive translation in murine ES cells
Ribosome profiling with translation inhibitors reveals pervasive translation in murine ES cells. Ribosome profiling or stranded RNAseq (ribominus) with murine embryonic stem cells treated with either DMD-pateamineA, Puromycin, Harringtonine or vehicle (no drug control). Two replicates per condition.
ORGANISM(S): Mus musculus 
2016-01-12 | E-GEOD-67741 | biostudies-arrayexpress
Sort   by:  
 Page size