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HeLa cells were fracitonated into nuclear soluble, chromatin bound and cytosolic. Extracts were immunoprecipitated with a CSN3 antibody, then eluted with glycine. Proteins were digested, then enriched for phosphopeptides. Samples were analyzed on an LTQ-Orbitrap. Data was analyzed using the TRansOMi...
ORGANISM(S): Homo sapiens (Human) 
2019-11-25 | PXD000277 | Pride
To assess the performance of computational methods for exon identification, transcript reconstruction and expression level quantification from RNA-seq data, 24 protocol variants of 14 independent software programs (AUGUSTUS, Cufflinks, Exonerate, GSTRUCT, iReckon, mGene, mTim, NextGeneid, Oases, SLI...
ORGANISM(S): Drosophila melanogaster 
RNA-seq transcript assembly evaluation
Gaucher disease, a recessive inherited metabolic disorder caused by defects in the gene encoding glucosylceramidase (GlcCerase), can be divided into three subtypes according to the appearance of symptoms associated with central nervous system involvement. We now identify a protein, glycoprotein non-...
ORGANISM(S): Homo sapiens (Human) 
2016-06-24 | PXD001654 | Pride
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