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TranscriptomicsSingle-cell RNA-seq (10x Chromium) of FACS-sorted drl:H2B-Dendra2, etv2:Kaede, fli1:DsRed, and tp1:eGFP transgenic zebrafish embryos
Development of the dorsal aorta is a key step in the establishment of the adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelia...
ORGANISM(S): Danio rerio 
2023-08-01 | E-MTAB-13196 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of 6 hours post fertilization mecp2-null versus wild type zebrafish embryos
RNA sequencing was performed on RNA isolated from groups of 6 hpf wild type and mecp2-null embryos (n=3 biological replicates per condition with 30 embryos pooled per replicate). DESeq2 analysis was performed using https://usegalaxy.org/ Whole embryo mRNA profile of 30 pooled mecp2-null or wild type...
ORGANISM(S): Danio rerio 
2016-09-01 | E-GEOD-80348 | biostudies-arrayexpress
TranscriptomicsMacrophage-specific gene functions in Spi1-directed innate immunity
The Spi1/ Pu.1 transcription factor plays a crucial role in myeloid cell development across many species. Several Spi1 target genes have been identified so far, yet the Spi1-dependent gene group remains largely unknown. To identify novel genes downstream of Spi1 we employed a microarray strategy usi...
ORGANISM(S): Danio rerio 
2010-04-30 | E-GEOD-19206 | biostudies-arrayexpress
TranscriptomicsGene expression changes in Itpr triple conditional knockout T cell progenitors
Calicum plays critical roles in developing T cells, promoting survival, proliferation, and differentiation at multiple stages of maturation. Calcium release from intracellular stores due to T cell receptor and pre-T cell receptor signaling is thought to require the inositol triphosphate receptors (I...
ORGANISM(S): Mus musculus 
2014-05-28 | E-GEOD-57997 | biostudies-arrayexpress
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