Macrophage-specific gene functions in Spi1-directed innate immunity

ABSTRACT: The Spi1/ Pu.1 transcription factor plays a crucial role in myeloid cell development across many species. Several Spi1 target genes have been identified so far, yet the Spi1-dependent gene group remains largely unknown. To identify novel genes downstream of Spi1 we employed a microarray strategy using zebrafish embryos. We established the gene group down-regulated upon spi1 knockdown while simultaneously enriched in FACS-sorted embryonic myeloid cells of a spi1:GFP transgenic line, thus representing putative myeloid-specific Spi1 target genes. This gene group contained all previously identified Spi1-dependent zebrafish genes, confirming the validity of the approach, as well as novel immune-related genes. Colocalization studies with neutrophil and macrophage markers revealed that genes cxcr

ORGANISM(S): Danio rerio

SUBMITTER: Anna Zakrzewska 

PROVIDER: E-GEOD-19206 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress