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TranscriptomicsTranscription profiling of human HCaRG-9 cells vs NEO-1 cells to identified differentially expressed genes between HCaRG9 and Neo1 cells
HEK293 cells were transfected with control plasmid (pcDNAI/Neo;Invitrogen) or with the plasmid encoding HCaRG. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen). Chips: HG-U133A; - Labeling protocol: Enzo ...
ORGANISM(S): Homo sapiens 
2007-09-07 | E-GEOD-2555 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HEK cells transfected with the calcium regulated gene HcARG reveals HCaRG increases renal cell migration by a TGF-alpha autocrine loop mechanism
HEK293 cells were transfected with control plasmid (pcDNA1/Neo; Invitrogen) or with the plasmid encoding HCaRG by a standard calcium phosphate co-precipitation method. The clones expressing the highest levels of HCaRG, HCaRG clone 8 and 9 were used in this experiment, while clone transfected with ve...
ORGANISM(S): Homo sapiens 
2007-11-11 | E-GEOD-4737 | biostudies-arrayexpress
ProteomicsThe lactococcal phage p2 protein ORF47 impedes Lactococcus lactis MG1363 resistance to phage infection
The lactococcal phage p2 is a model for studying the Skunavirus genus, the most prevalent group of phages in cheese factories worldwide. It infects L. lactis MG1363, a model strain for the study of Gram-positive bacteria. The structural proteins of phage p2 have been thoroughly described. However, m...
ORGANISM(S): Lactococcus lactis subsp. cremoris MG1363 Lactococcus phage 
2020-07-30 | PXD013186 | Pride
Lactococcus lactis Label-free quantification Mass spectrometry-based proteomics Phage infection 
TranscriptomicsTracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: a Link Between Stalled Polyribosomes and RNA Granules
Local translation at the synapse plays key roles in neuron development and activity-dependent synaptic plasticity. mRNAs are translocated from the neuronal soma to the distant synapses as compacted ribonucleoparticles referred to as RNA granules. These contain many RNA-binding proteins, including th...
ORGANISM(S): Mus musculus 
2016-06-30 | E-GEOD-83835 | biostudies-arrayexpress
TranscriptomicsGene expression profile of calcified and normal tricuspid aortic valves by RNA sequencing.
Purpose: Calcific aortic valve stenosis (AS) is a fatal disease with currently no medical therapy. Some genes were associated with AS, but the genetic architecture of the disease has yet to be discovered. The objective of this study was to combine genome-wide association studies (GWAS) and gene expr...
ORGANISM(S): Homo sapiens 
2015-12-09 | E-GEOD-55492 | biostudies-arrayexpress
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