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TranscriptomicsNgn2 induces diverse neuronal lineages from human pluripotency
Human neurons engineered from induced pluripotent stem cells (iPSCs) through Neurogenin 2 (Ngn2) overexpression are widely used to study neuronal differentiation mechanisms and to model neurological diseases. However, the differentiation paths and heterogeneity of emerged neurons have not been fully...
ORGANISM(S): Homo sapiens 
2021-08-05 | E-MTAB-10632 | biostudies-arrayexpress
TranscriptomicsMosaic perturbation of transcription factors in human retinal organoid development
To begin to understand how TFs regulate retinal cell type identity in human tissues, we established a pooled loss of function (LOF) experiment based on the CROP-seq protocol in developed retinal organoids. We targeted five TFs (OTX2, NRL, CRX, VSX2, and PAX6) that are important for retinal developme...
ORGANISM(S): Homo sapiens 
2023-02-24 | E-MTAB-12622 | biostudies-arrayexpress
TranscriptomicsSingle-cell chromatin accessibility atlas of human retinal organoid development
In order to provide multi-omic resolution to human retinal organoid developmental dynamics, we performed scRNA-seq and scATAC-seq from the same cell suspension across a time course (6-46 weeks) of human retinal organoid development. This data set covers all the retinal organoid scATAC-seq data gener...
ORGANISM(S): Homo sapiens 
2023-03-12 | E-MTAB-12714 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptome atlas of human retinal organoid development
In order to provide multi-omic resolution to human retinal organoid developmental dynamics, we performed scRNA-seq and scATAC-seq from the same cell suspension across a time course (6-46 weeks) of human retinal organoid development. This data set covers all the retinal organoid scRNA-seq data genera...
ORGANISM(S): Homo sapiens 
2023-03-12 | E-MTAB-12715 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptome of cerebral organoids with and without methanol fixation
To study how methanol fixation affects single-cell transcriptomic measurement, two cerebral organoids were dissociated. Cell suspension of each organoid was split into two aliquots. Methanol fixation was applied to one of the two aliquots. Single-cell RNA-seq with 10x Genomics was applied to the two...
ORGANISM(S): Homo sapiens 
2020-08-31 | E-MTAB-9473 | biostudies-arrayexpress
TranscriptomicsLineage recording in human cerebral organoids (Visium)
Induced pluripotent stem cell (iPSC) derived organoid systems provide models to study human organ development. Single-cell transcriptome sequencing enables highly-resolved descriptions of cell state heterogeneity within these systems and computational methods can reconstruct developmental trajectori...
ORGANISM(S): Homo sapiens 
2021-11-03 | E-MTAB-10972 | biostudies-arrayexpress
TranscriptomicsLineage recording in human cerebral organoids (microdissected)
Induced pluripotent stem cell (iPSC) derived organoid systems provide models to study human organ development. Single-cell transcriptome sequencing enables highly-resolved descriptions of cell state heterogeneity within these systems and computational methods can reconstruct developmental trajectori...
ORGANISM(S): Homo sapiens 
2021-11-03 | E-MTAB-10973 | biostudies-arrayexpress
TranscriptomicsLineage recording in human cerebral organoids (iTracer-perturb)
Induced pluripotent stem cell (iPSC) derived organoid systems provide models to study human organ development. Single-cell transcriptome sequencing enables highly-resolved descriptions of cell state heterogeneity within these systems and computational methods can reconstruct developmental trajectori...
ORGANISM(S): Homo sapiens 
2021-11-03 | E-MTAB-10971 | biostudies-arrayexpress
TranscriptomicsLineage recording in human cerebral organoids (whole organoids)
Induced pluripotent stem cell (iPSC) derived organoid systems provide models to study human organ development. Single-cell transcriptome sequencing enables highly-resolved descriptions of cell state heterogeneity within these systems and computational methods can reconstruct developmental trajectori...
ORGANISM(S): Homo sapiens 
2021-11-03 | E-MTAB-10974 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA sequencing of developing human neural organoids exposed to an array of morphogen treatments varying morphogen concentration, timing and combinatorics
This experiment seeks to unravel the effects of concentration, timing and combinatorial variables on the response to morphogens during early human neural organoid development. Human neural organoids derived from three different hESC lines were cultured individually and exposed to systematic variatio...
ORGANISM(S): Homo sapiens 
2025-10-15 | E-MTAB-15667 | biostudies-arrayexpress
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