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ProteomicsPrecision-edited histone tails perturb polycistronic gene expression controls in trypanosomes
Transcription of protein coding genes in trypanosomatids is atypical and almost exclusively polycistronic. In Trypanosoma brucei, approximately 150 polycistrons, and 8000 genes, are constitutively transcribed by RNA polymerase II, while a smaller subset can be transcribed by RNA polymerase I. RNA po...
ORGANISM(S): Trypanosoma brucei 
2025-03-11 | PXD061709 | Pride
Trypanosomes h4 t. brucei polycistronic expression control 
TranscriptomicsRNAi knock-down of VSG in T. brucei for 1 or 3 days
As an additional strategy for investigating T. brucei transcriptome responsiveness, the mRNA of a highly important protein, the variant surface glycoprotein (VSG) the major surface protein in the bloodstream stage, was suppressed with RNAi. VSG RNAi results in arrest of cell cycle progression...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1528 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei in vivo or in vitro samples, with in vitro samples either inclubated at 4oC or not to investiage the influence of culturing condtions on intracellular transport
The vast majority of molecular studies on T. brucei are carried out using in vitro cultures. Recently, whole-genome microarray analysis indicated significant differences between in vivo and in vitro cultures of the malaria parasite Plasmodium falciparum. Therefore, as an additional investigation int...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1540 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei bloodstream and procyclic forms to look at differential expression of membrane-trafficking genes
To look at differential expression of membrane-trafficking genes,
we extracted RNA from exponentially growing cultures of T. brucei
bloodstream (BSF) and procyclic (PCF) forms, labelled and competitively
hybridised the cDNA to the microarray. 8 arrays, representing
6 BSF and 5 PCF bi...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1529 | biostudies-arrayexpress
no omics definedTranscription profiling of T. brucei treated with dithiothreitol (DTT) or tunicamycin to invoke the unfolded protein response
Transcriptome responsiveness was further tested by attempts to invoke the unfolded protein repsonse (UPR), a classic ER-based pathway stimulated by the presence of increased levels of unfolded polypeptides. The UPR is mediated via transcriptional responses in both yeast and metazoan cells, and can b...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1533 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei bloodstream forms cultured in different proportions of fetal bovine serum to investigate iron-sensing mechanisms
Bloodstream trypanosomes are sensitive to iron starvation, and upregulate ESAG6/7 transferrin receptor (TfR) mRNA and protein levels when placed in low transferrin or iron-depleted media, and adjust TfR expression to compensate for reduced endocytosis. Both observations suggest a specific transcript...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1530 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei cell lines over-expressing wild type RAB5A or mutant versions of RAB5A or RAB5B locked in the GTP-bound state
As a test of trypanosome transcriptome responsiveness, we analysed the effects of over-expression of the early endosome small GTPase Rab5A in procyclic form T.brucei, which results in significant augmentation of fluid phase and receptor-mediated endocytic activity (Pal et al. 2002).

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ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1539 | biostudies-arrayexpress
Genomics
Precision-edited histone tails perturb polycistronic gene expression controls in trypanosomes
PRJNA1234166 | ENA
TranscriptomicsWild-type Lister 427 Bloodstream-form polysome profiles with ZC3H32 knock-down
Ran from polysomes of Lister 427 bloodstream forms, wild-type and a cell line with a double knockout and inducible ZC3H32, grown without tetracycline.
ORGANISM(S): Trypanosoma brucei 
2017-11-24 | E-MTAB-4558 | biostudies-arrayexpress
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