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TranscriptomicsscRNA-seq of intestinal epithelial organoids derived from human fetal duodenum
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of EGF or 1 ng/ml of EREG added.
ORGANISM(S): Homo sapiens 
2022-07-17 | E-MTAB-11912 | biostudies-arrayexpress
TranscriptomicsSmall Perturbation Approach Reveals Transcriptomic Steady State
The â??small perturbationâ?? approach is critical in studying the â??steady stateâ?? of a biological system. In our experiments, small perturbations were generated by applying a series of repeating intermittent small doses of ultraviolet radiation to a human keratinocyte cell line, HaCaT. The biolog...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-7060 | biostudies-arrayexpress
TranscriptomicsSingle-nucleus RNA sequencing of positional airway organoids derived from human fetal lung at 4 anatomic regions (trachea, bronchus, NCA, distal)
We derived primary, epithelial-enriched organoids from 120 PCD human fetal lung, specifically from trachea, bronchus, non-cartilaginous airway, and distal airway. These positional airway organoids were grown in FANY airway media for the duration of 2 passages before collection for single-nucleus RNA...
ORGANISM(S): Homo sapiens 
2025-08-01 | E-MTAB-14961 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human epithelium-only colonic and small intestinal organoids derived from intestinal resections, biopsy specimens and cryopreserved crypts
Patient-derived gastrointestinal epithelium-only organoids from the small and large intestine, also referred to as colonoids and enteroids, were generated as part of the development of an on-going organoid biobank at the Michigan Medicine Translational Tissue Modeling Laboratory (TTML) (www.UmichTTM...
ORGANISM(S): Homo sapiens 
2020-07-24 | E-MTAB-9339 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of CellTagging lung organoids
Here, we used a lentiviral-based barcoding method (CellTagging) to lineage trace progenitor cells in a human lung organoid model. 21-day cultures were transduced for 7 days, fluorescence activated cell sorted for GFP+ expression, and regrown as sparsely plated cells for additional 30 days before seq...
ORGANISM(S): Homo sapiens 
2023-04-21 | E-MTAB-12753 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of hPSC-derived human intestinal organoids with endogenous vascular endothelial cells
The development of human pluripotent stem cell (hPSC)- derived small intestinal organoids (HIOs) has led to an improved understanding of human intestinal development and physiology. HIOs generated using directed differentiation lack some cellular populations found in the native organ, including vasc...
ORGANISM(S): Homo sapiens 
2020-12-20 | E-MTAB-9228 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal lung and intestine tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of 1 (one) human fetal lung tissue specimen (18.9 week) and 2 (two) human fetal intestine specimens (12.1 and 18.9 week) (total of 3 (three) independent biological specimens). The data set is composed of approximately 5,000 lung cells and 7,5...
ORGANISM(S): Homo sapiens 
2020-12-20 | E-MTAB-9363 | biostudies-arrayexpress
TranscriptomicsscRNAseq of human fetal duodenum, 70 DPC
We performed single cell RNA sequencing (scRNA-seq) of human fetal duodenal tissue samples from a single individual biological specimen at 70 days post-conception (DPC). The data set is composed of cells from diverse intestinal lineages. Lineages captured include epithelium, mesenchyme, immune, neur...
ORGANISM(S): Homo sapiens 
2025-07-30 | E-MTAB-15358 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids grown in an alginate matrix
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in an alginate matrix after 3, 7, and 14 days of in vitro growth. Samples were grown in minigut media supplemented with either ENR or EGF.
ORGANISM(S): Homo sapiens 
2022-02-01 | E-MTAB-11338 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal kidney tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal kidney tissue samples from 2 individual biological specimens (13.7 and 15.4 weeks gestation). The data set is composed of approximately 10,000 cells from diverse renal lineages. Lineages captured include nephron progenitors, ep...
ORGANISM(S): Homo sapiens 
2020-12-20 | E-MTAB-9083 | biostudies-arrayexpress
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