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TranscriptomicsHCT116 cells isogenic for p53 expression upon treatment with actinomycin D and nutlin-3
HCT116 wild type cells express p53. The isogenic HCT116 p53KO cells have the p53 gene knocked out. Cells were treated for 16 hours. Dosages for actinomycin D treatment was 1 nM, 10 nM, and 100 nM. Dosage for nutlin-3 was 4 uM. Keywords: dose response Measuring the gene expression profile of the is...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-12459 | biostudies-arrayexpress
GenomicsLight dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn
Light dependent gene expression in D. shibae wildtype compared to the gene expression in the transposonmutants D. shibae Dshi_1135::Tn, D. shibae ppaA::Tn and D. shibae ppsR::Tn
PRJNA1022088 | ENA
ProteomicsMetabolic exploration of L-lactate- and D-lactate-consuming microbial communities.
Metabolic exploration of L-lactate- and D-lactate-consuming microbial communities.
ORGANISM(S): Propionibacteriaceae 
2026-01-27 | PXD068334 | Pride
Microbial community L/d lactate utilisation 
TranscriptomicsTU-tagging: cell type specific RNA isolation from intact complex tissues
We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to i...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-15445 | biostudies-arrayexpress
TranscriptomicsGenomewide measurements of whole embryo mRNA decay in Drosophila
whole embryo (all tissues) measurement of mRNA decay by 4-thiouridine pulse-chase These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Measurements with or without transcription inhibition by actinomycin D (ActD) were compared.
ORGANISM(S): Drosophila melanogaster 
2015-03-31 | E-GEOD-67435 | biostudies-arrayexpress
UnknownOutwitting EF-Tu and the ribosome: translation with d-amino acids.
Not available
S-EPMC4499158 | biostudies-literature
ProteomicsSalmonella antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of EF-Tu P-loop
Rearrangement hot spots (Rhs) proteins are members of the broad family of polymorphic toxins. Polymorphic toxins are modular proteins composed of an N-terminal region that specifies their mode of secretion into the medium or into the target cell, a central delivery module and a C-terminal domain tha...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Bacteria 
2023-03-11 | PXD033100 | Pride
Elongation factor Adpribosylation Toxin Rearrangement hot spots T6ss P-loop Modification Translation 
UnknownA protein extension to shorten RNA: elongated elongation factor-Tu recognizes the D-arm of T-armless tRNAs in nematode mitochondria.
Not available
S-EPMC1609916 | biostudies-literature
MetabolomicsEnzyme engineering for optimizing biosynthesis of 2,4-dihydroxybutyric acid via the synthetic threose-dependent glycolaldehyde assimilation (STEGA) pathway
Ethylene glycol is a potential feedstock for next-generation biorefineries, as it can be derived from both plastic waste and carbon dioxide. The synthetic D-threose-dependent glycolaldehyde assimilation (STEGA) pathway, which is orthogonal to central metabolism of Escherichia coli, was recently cons...
2026-07-15 | MTBLS15050 | MetaboLights
UnknownElongation Factor Tu Prevents Misediting of Gly-tRNA(Gly) Caused by the Design Behind the Chiral Proofreading Site of D-Aminoacyl-tRNA Deacylase.
Not available
S-EPMC4880308 | biostudies-literature
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