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In this study we performed 2 interactomes. To identify interactants of RNF111 that are dependent of the RING domain, we performed qualitative interactome comparison of HEK-293 cells transfected with GFP, GFP-RNF111-wt or GFP-RNF11-C933A mutated in its RING domain. This led to the identification of U...
ORGANISM(S): Homo sapiens (Human) 
2023-04-07 | PXD034999 | Pride
Transcriptional profiling of esg+-midgut progenitor cells expressing esgRNAi or UAS-esg AND of whole midgut tissue from flies overexpressing UAS-esg in enterocyte (EC) cells
Drosophila melanogaster strain:Gr66aGal4/UAS-H2B YFP (TOG)/ Orco Gal4/UAS-H2B YFP (DOG) Transcriptome or Gene expression
RNA-Seq of amplified mRNA from sorted esg+-midgut progenitor cells expressing esgRNAi or UAS-esg AND RNA-Seq of whole midgut tissue from flies overexpressing UAS-esg and UAS-esg+UAS-dIAP (+control empty vector RNAi, UAS-dIAP and w1118) using the MyoIA-Gal4ts EC-driver
ORGANISM(S): Drosophila melanogaster 
Transcriptome analysis of the targeted overexpression of branchless (UAS-bnl) using a tracheal driver (breathless-GAL4) in comparison to a wildtype reference at two different developmental stages (10-11h AEL and 17-19h AEL), and of a bnl-P1 mutant in comparison to a wildtype reference at 17-19h AEL.
ORGANISM(S): Drosophila melanogaster 
Large-scale reporter assay to assess expression and coactivator specificity of UAS-core promoter combinations [III]
Large-scale reporter assay to assess expression and coactivator specificity of UAS-core promoter combinations (RNA)
Large-scale reporter assay to assess expression and coactivator specificity of UAS-core promoter combinations (DNA)
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