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ProteomicsIdentification of ubiquitin interactors in Legionella pneumophila using ubiquitin activity-based probes
In this experiments, we used several ubiquitin activity-based probes (Ub-Prg, Ub-VME, and Ub-VS) to covalently capture ubiquitin-interacting proteins in Legionella pneumophila. These enriched interactome were analyzed by LC-MS/MS to identify Legionella effectors uniquely captured by the probes.
ORGANISM(S): Legionella Pneumophila Subsp. Pneumophila Lp02 (ncbitaxon:1080311) 
2023-12-09 | MSV000093623 | MassIVE
ProteomicsDiscovery of highly Potent and Selective Activity-Based Probes for the Deubiquitylating Enzyme USP30
Ubiquitin-specific protease 30 (USP30) is a deubiquitylating enzyme (DUB) localized in the mitochondrial membrane, which is related to PINK1/Parkin-mediated mitophagy, pexophagy, BAX/BAK-dependent apoptosis, and IKKβ-USP30-ACLY-regulated lipogenesis/tumorigenesis. Mission therapeutics pointed their ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD044792 | Pride
Usp30  activity-based probes  chemical probes  desi  deubiquitinase. 
ProteomicsGenetically encoded activity-based ubiquitin and ubiquitin-like protein covalent probes
The nucleophilic thiol-alkyne addition reaction can form a covalent thiovinyl bond with cysteine reactivity specific,as the classical standards for cysteine-reactive warheads in activity-based probes (ABPs).Utilizing this classical reactivity property, we designed two potentially reactive unnatural...
ORGANISM(S): Homo Sapiens 
2025-05-14 | PXD063756 |
ProteomicsActivity-based profiling of cullin-RING ligase networks by conformation-specific probes
The cullin-RING E3 ligase (CRL) network comprises over 300 unique complexes that switch from inactive to activated conformations upon site-specific cullin modification by the ubiquitin-like protein NEDD8. Assessing cellular repertoires of activated CRL complexes is critical for understanding eukaryo...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-09-02 | PXD039649 | Pride
Ligase Cullin Nedd8 Ring Ubiquitin E3 
ProteomicsDevelopment of covalent probes to capture Legionella pneumophila effector enzymes
Upon infection of host cells Legionella pneumophila releases a multitude of effector enzymes into the hosts cytoplasm that hijack a plethora of cellular activities, including the hosts ubiquitination pathways. Effectors belonging to the SidE-family are involved in non-canonical serine phosphoribosyl...
ORGANISM(S): Homo sapiens (Human) Legionella pneumophila subsp. pneumophila Lp02 
2025-05-06 | PXD049797 | Pride
Legionella pneumophila Activity-based probe Ubiquitylation Adp-ribosylation Bacterial infection 
ProteomicsGenetically Encoded Activity-Based Ubiquitin and Ubiquitin-like Protein Probes
Post-translational modification of proteins by Ubiquitin (Ub) and Ubiquitin-like proteins (Ubls) can be reversed by deconjugating enzymes, which have been implicated in different pathways and associated with various human diseases. To understand the activity and dynamics of deconjugating enzymes, mu...
ORGANISM(S): Homo Sapiens 
2022-09-23 | PXD036955 |
ProteomicsPhotocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions
Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans. RING E3s facilitate transfer of ubiquitin to substrates by...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075613 | Pride
Ubiquitin n-maleimido-diazirine e3 ligase activity-based probe 
ProteomicsIdentification of novel ubiquitin receptors on the 26S proteasome by photo-crosslinking mass spectrometry
The 26S proteasome is the endpoint of the ubiquitin-proteasome system, an essential pathway for maintaining cellular homeostasis through targeted degradation of misfolded, damaged, and obsolete proteins. Substrates labeled with ubiquitin are directed to the 26S proteasome by binding to one or more u...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-06-08 | PXD075114 | Pride
26s proteasome Ubiquitin Ubiquitin receptors 
UnknownActivity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.
Not available
S-EPMC7163952 | biostudies-literature
UnknownPhotocrosslinking Activity-Based Probes for Ubiquitin RING E3 Ligases.
Not available
S-EPMC6963778 | biostudies-literature
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