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TranscriptomicsE2-RING expansion of the NEDD8 cascade confers specificity to cullin modification
Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated...
ORGANISM(S): Mus musculus 
2009-03-23 | E-GEOD-14088 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of myeloma cells treated with NAE inhibitor
The transcriptional profile of the human multiple myeloma (MM) cell line MM.1S treated with MLN4924 vs control MM.1S cells was characterized by oligonucleotide microarray analysis, using the human U133 plus 2.0 Affymetrix GeneChip, according to previously described protocols for total RNA extraction...
ORGANISM(S): Homo sapiens 
2012-05-01 | E-GEOD-33577 | biostudies-arrayexpress
ProteomicsInteractome of S65 phosphorylated NEDD8 and Ub
In this project we present for the first time a proteome-wide interaction study of S65 phosphorylated NEDD8 and ubiquitin (Ub). By using cell extracts and affinity enrichment coupled to mass spectrometry, we show that pNEDD8 and pUb have different interactomes that are also distinct from those of th...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD021143 | Pride
Phosphorylation Nedd8 Ubiquitin Interactome 
ProteomicsNEDD8 S65 phosphorylation under different stress conditions
In this project we present the identification of NEDD8 S65 phosphorylation under mitochondrial and DNA double strand break (DSB) stress conditions. Relative comparison revealed elevated levels of pNEDD8 under mitochondrial stress and reduced levels of pNEDD8 under DSB stress.
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD027477 | Pride
Stress Phosphorylation Nedd8 
ProteomicsMCF-7 PTEN-Nedd8 IP-MS
We intended to elucidate the underlying mechanism of PTEN-Nedd8 in tumorigenesis. So, we firstly employed affinity purification and mass spectrometry to interrogate PTEN-Nedd8 interactome in nucleus.
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD011395 | Pride
Human Breast cancer cell Nedd8 Pten Ip-ms 
ProteomicsSILAC diGlycine proteomics in C. elegans to identify ULP-3 targets upon DNA damage.
We found a conserved role for the deNEDDylating enzyme NEDP1 (ULP-3 in C. elegans) in the DNA damage induced apoptosis. To gain mechanistic insights into the role of ULP-3 in the IR-induced apoptosis, we devised an unbiased proteomics approach to discover potential NEDD8 targets for ULP-3 upon DNA d...
ORGANISM(S): Nematodes (ncbitaxon:333870) 
2019-08-19 | MSV000084214 | MassIVE
ProteomicsCharacterization of Plasmodium falciparum NEDD8
Here, we characterize the Plasmodium falciparum NEDD8 and demonstrate that P. falciparum cullins are NEDD8 substrates. This work lays the foundation for understanding the role of protein neddylation in malaria parasites.
ORGANISM(S): Plasmodium falciparum D10 
2021-09-09 | PXD020952 | Pride
Plasmodium Neddylation Nedd8 
ProteomicsNEDD8 subtrates from NEDP1 knockout cells
The aim of the study was to identify proteins that are modified by NEDD8. The NEDD8 specific protease NEDP1/SENP8 was deleted from HEK 293 cells via Crispr/CAS9 gene editing to allow for the accumulation of NEDD8 modified proteins. Lysates from NEDP1 KO cells were then enriched via pulldown with a...
ORGANISM(S): Homo sapiens (Human) 
2019-02-20 | PXD011928 | Pride
Human Nedd8 Nedp1 Senp8 Hek 293 
ProteomicsCharacterization of Plasmodium falciparum NEDD8
Here, we characterize the Plasmodium falciparum NEDD8 and demonstrate that P. falciparum cullins are NEDD8 substrates. This work lays the foundation for understanding the role of protein neddylation in malaria parasites.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-08 | PXD020885 | Pride
Ubiquitin-like modifier Malaria Neddylation Nedd8 Ubiquitin Cullin 
ProteomicsProteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation
The Ubiquitin-like molecule NEDD8 controls several biological processes and is a promising target for therapeutic intervention. NEDDylation occurs through specific NEDD8 enzymes (canonical) or enzymes of the Ubiquitin system (atypical). Identification of NEDD8 sites on substrates is critical for del...
ORGANISM(S): Homo sapiens (Human) 
2021-02-10 | PXD022812 | Pride
Proteomics Sumo-2 Nucleolus-related inclusions Digly remnants Nedd8 Nedd8 polymers 
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