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ProteomicsNEDD8 subtrates from NEDP1 knockout cells
The aim of the study was to identify proteins that are modified by NEDD8. The NEDD8 specific protease NEDP1/SENP8 was deleted from HEK 293 cells via Crispr/CAS9 gene editing to allow for the accumulation of NEDD8 modified proteins. Lysates from NEDP1 KO cells were then enriched via pulldown with a...
ORGANISM(S): Homo sapiens (Human) 
2019-02-20 | PXD011928 | Pride
Human Nedd8 Nedp1 Senp8 Hek 293 
ProteomicsIdentification of NEDD8 sites at endogenous levels of NEDD8 expression upon NEDP1 (SENP8, DEN1) knockout.
We developed a strategy that allows the identification of NEDP1 dependent NEDDylation sites under endogenous expression of wild type NEDD8. We combined the use of anti-diGly antibodies that recognise both ubiquitin and NEDD8 modified peptides upon trypsin digestion with short treatment of cells with...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD040466 | Pride
Antibody Digly Inhibitor Nedd8 Neddylation Uaei Anti-digly antibodies Ubiquitin 
UnknownStructural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1.
Not available
S-EPMC1142549 | biostudies-literature
UnknownTargeting the NEDP1 enzyme to ameliorate ALS phenotypes through stress granule disassembly.
Not available
S-EPMC10065448 | biostudies-literature
ProteomicsSILAC diGlycine proteomics in C. elegans to identify ULP-3 targets upon DNA damage.
We found a conserved role for the deNEDDylating enzyme NEDP1 (ULP-3 in C. elegans) in the DNA damage induced apoptosis. To gain mechanistic insights into the role of ULP-3 in the IR-induced apoptosis, we devised an unbiased proteomics approach to discover potential NEDD8 targets for ULP-3 upon DNA d...
ORGANISM(S): Nematodes (ncbitaxon:333870) 
2019-08-19 | MSV000084214 | MassIVE
UnknownThe Balance between Mono- and NEDD8-Chains Controlled by NEDP1 upon DNA Damage Is a Regulatory Module of the HSP70 ATPase Activity.
Not available
S-EPMC6899524 | biostudies-literature
UnknownNEDDylation antagonizes ubiquitination of proliferating cell nuclear antigen and regulates the recruitment of polymerase η in response to oxidative DNA damage.
Not available
S-EPMC5876183 | biostudies-literature
UnknownUnanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.
Not available
S-EPMC6418418 | biostudies-literature
ProteomicsAntibody RING-Mediated Destruction (ARMeD) of endogenous proteins
To understand the cellular function of a gene the encoding DNA or mRNA transcript can be manipulated and the consequences observed. However, these approaches do not have a direct effect on the protein product of the gene, which is either permanently abrogated or depleted at a rate defined by the hal...
ORGANISM(S): Homo sapiens (Human) 
2020-05-29 | PXD018113 | Pride
Ring ubiquitin nanobody endogenous degradation 
UnknownNeddylation of PTEN regulates its nuclear import and promotes tumor development.
Not available
S-EPMC8027835 | biostudies-literature
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