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ProteomicsAdaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation
Partitioning of active gene loci to the nuclear envelope is a key mechanism by which organisms can increase the speed of adaptation and metabolic robustness to fluctuating resources in the environment. In the budding yeast Saccharomyces cerevisiae, adaptation and transcriptional memory induced by nu...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-01 | PXD029913 | Pride
Active gene Nuclear envelope Ino1 Gene partitioning Chromatin decompaction Active chromatin Phase separation 
UnknownSpherical covalent organic frameworks as advanced adsorbents for preconcentration and separation of phenolic endocrine disruptors, followed by high performance liquid chromatography.
Not available
S-EPMC9083371 | biostudies-literature
MetabolomicsMetabolic and gut microbial remodeling in captive Amur tigers under uniform feeding

Ultra-high-performance liquid chromatography (UHPLC) was conducted using a Vanquish UHPLC system (Thermo Fisher), coupled with a Q Exactive™ HF/Q Exactive™ HF-X mass spectrometer for liquid chromatography-mass spectrometry (LC-MS) analysis. To ensure...

2026-06-05 | MTBLS14159 | MetaboLights
ProteomicsCapillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-MS/MS) for ultra-sensitive proteomic analysis of limited sample
In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 pg and 88 pg of HeLa protein digest standard by CE-MS/MS yielded ~1,100±46 and ~160±59 proteins, respectively, demonstrati...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027226 | Pride
Ultra-low flow liquid phase separation Ce-ms Ultra-sensitive proteomics Monolithic columns Capillary electrophoresis Rp-nlc-ms 
ProteomicsProteomic analysis of Mycoplasma hyopneumoniae virulent strain 232
The experiment consists of M.hyp 232 cultures digests analyzed on two mass specs, LTQ Velos Pro and LTQ FT Ultra LTQ Velos Pro: Six cell culture replicates of M.hyo 232 were hydrophobically separated using tree detergents: Digitonin, Tween, and SDS. Each francion, including the insoluble pellet, wa...
ORGANISM(S): Mycoplasma hyopneumoniae (strain 232) 
2018-01-10 | PXD000118 | Pride
Mycoplasma hyopneumoniae 232 ltq velos ft mass spec proteomics 
UnknownDevelopment of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies.
Not available
S-EPMC3110015 | biostudies-literature
MetabolomicsA Comprehensive and Ultrasensitive Isotope Calibration Method for Soil Amino Compounds Using Orbitrap Mass Spectrometry
Bound amino compounds (amino acid and amino sugar polymers) comprise a significant proportion (~40%) of soil organic nitrogen and therefore represent an essential source of nitrogen for plant and microbial nutrition. The analysis of their content and isotope enrichment still represents a significant...
2025-06-16 | MTBLS12550 | MetaboLights
MetabolomicsStudy on the Hepatoprotection of Schisandra chinensis Caulis Ploysaccharides in nonalcoholic fatty liver disease in rats based on metabolomics

The aim of this study was to investigate the hepatoprotection of Schisandra chinensis Caulis polysaccharides (SCP) in the nonalcoholic fatty liver disease (NAFLD) induced by high-fat diet (HFD) in rats. A total of 30 Wistar rats were randomly divided into control group (CON), model group...

2021-08-24 | MTBLS3194 | MetaboLights
UnknownDevelopment of a Rapid UHPLC-PDA Method for the Simultaneous Quantification of Flavonol Contents in Onions (Allium cepa L.).
Not available
S-EPMC8066725 | biostudies-literature
MetabolomicsMethod Comparison using DBS samples

Metabolomics serves as a critical pillar in clinical research, yet the lack of standardized protocols often limits the comparability of data across different platforms and institutions. The HUMAN (Harmonizing and Unifying Blood Metabolomics Network) has as one of its goals to to advance the field...

2026-05-19 | MTBLS14519 | MetaboLights
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