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ProteomicsCapillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-MS/MS) for ultra-sensitive proteomic analysis of limited sample
In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 pg and 88 pg of HeLa protein digest standard by CE-MS/MS yielded ~1,100±46 and ~160±59 proteins, respectively, demonstrati...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027226 | Pride
Ultra-low flow liquid phase separation Ce-ms Ultra-sensitive proteomics Monolithic columns Capillary electrophoresis Rp-nlc-ms 
ProteomicsGeneration of the HCC spectral library covering more than 10,000 protein groups
Peptides of HCC cell lines and tumour tissues were fractionated by Hp-RP to eight fractions and repeated analysed three times by the LC-MS/MS detection system consisted of the EASY-nLC 1000 coupled to the Q-Exactive HF mass spectrometer using data dependent acquisition mode.
ORGANISM(S): Homo sapiens (Human) 
2022-11-11 | PXD035028 | Pride
Hcc proteome 
UnknownNovel ophthalmic formulation of myriocin: implications in retinitis pigmentosa.
Not available
S-EPMC6419690 | biostudies-literature
UnknownCapillary Electrophoresis Coupled to Electrospray Ionization Tandem Mass Spectrometry for Ultra-Sensitive Proteomic Analysis of Limited Samples.
Not available
S-EPMC8770592 | biostudies-literature
UnknownProtein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones).
Not available
S-EPMC8717388 | biostudies-literature
ProteomicsProtein mass spectrum analysis of gastrocnemius from CKD and NC mice
As for gastrocnemius (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionation with reversed-phase (RP) chromatography and mass spectrometr...
ORGANISM(S): Mus Musculus 
PXD055326 |
ProteomicsAnalysis of Erythroleukemia
Samples were subjected to LC–MS analysis using a dual pressure LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) connected to an electrospray ion source (Thermo Fisher Scientific) as recently described (PMID: 23017020). Peptide separation was carried out on an EASY nLC-1000 system (The...
ORGANISM(S): Homo sapiens (Human) 
2022-02-24 | PXD012490 | Pride
Erythroleukemia Mass spectroemtry 
ProteomicsPhosphoproteomic characterization of infulenza A virus infected human macrophages
Human primary macrophages were infected with influenza A virus (H3N2/Udorn strain, HA 256) for 6 hrs or left untreated. Cells were collected and lysed with HEPES lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 % NP-40, pH 7.4) including protease and phosphatase inhibitor cocktails. The cell lys...
ORGANISM(S): Homo sapiens (Human) 
2016-08-08 | PXD001620 | Pride
Primary human macrophages Phosphorylation Influenza a virus 
ProteomicsProtein mass spectrum analysis of serum from CKD and NC mice
As for serum (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. We utilized immunoaffinity depletion of serum high-abundance proteins. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionati...
ORGANISM(S): Mus Musculus 
PXD055324 |
ProteomicsFunctional identification of protocadherin alpha 9 (PCDHA9) as a candidate causative gene for amyotrophic lateral sclerosis
Mice were sacrificed after anesthesia by isoflurane following neck cutting. The spinal cord L3-L5 was taken and frozen in liquid nitrogen immediately and the ventral horn of the bilateral spinal cord (about 400um thick in gray matter area) was collected at low temperature. The samples were digested ...
ORGANISM(S): Mus Musculus 
PXD042929 |
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