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ProteomicsT7 DNA Polymerase with fluorescent unnatural amino acid
A T7 DNA polymerase variant with the fluorescent unnatural amino acid (7-hydroxycoumarin-4-yl) ethylglycine (7-HCou) incorporated at position 514 was expressed in E. coli and purified. The protein was then characterized by mass spec, pre-steady state kinetic experiments, and fluorescence measurement...
ORGANISM(S): Escherichia virus T7 
2020-10-19 | PXD021676 | Pride
T7 Unnatural amino acid Dna polymerase 7-hydroxycoumarin-4-yl ethylglycine 
UnknownTransferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency.
Not available
S-EPMC7822784 | biostudies-literature
ProteomicsUncover New Reactivity of Genetically Encoded Alkyl Bromide Unnatural Amino Acids
Genetically encoded unnatural amino acids (Uaas) with electrophilic moieties are excellent tools to investigate protein-protein interactions (PPIs) both in vitro and in vivo. These Uaas, including a series of alkyl bromide-based Uaas, mainly target cysteine residues to form protein-protein cross-lin...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-04-04 | PXD031159 | Pride
Chemical cross-linking And sumo interactome Unnatural amino acid Protein-protein interactions Genetic code expansion 
UnknownA remarkable activity of human leukotriene A4 hydrolase (LTA4H) toward unnatural amino acids.
Not available
S-EPMC3984412 | biostudies-literature
UnknownInteins as traceless purification tags for unnatural amino acid proteins.
Not available
S-EPMC4473854 | biostudies-literature
TranscriptomicsGenetic code expansion in stable cell lines enables encoded chromatin modification
Genetically encoded unnatural amino acids provide powerful strategies for modulating the molecular functions of proteins in mammalian cells. However this approach has not been coupled to genome-wide measurements, because efficient unnatural amino acid incorporation is limited to readily transfect...
ORGANISM(S): Mus musculus 
2016-07-03 | E-GEOD-73823 | biostudies-arrayexpress
UnknownTrifluoroselenomethionine: A New Unnatural Amino Acid.
Not available
S-EPMC5373900 | biostudies-literature
UnknownDirected Evolution of a Designer Enzyme Featuring an Unnatural Catalytic Amino Acid.
Not available
S-EPMC6519144 | biostudies-literature
UnknownEnhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.
Not available
S-EPMC4059402 | biostudies-literature
ProteomicsRab5a-VPS34 unnatural amino acid mediated crosslinking
The small GTPase Rab5 has an essential role in sorting vesicles arriving to and leaving the early endosome. One key function of Rab5 in this process is the activation of the primordial phosphatidylinositol 3-kinase VPS34, a lipid kinase that phosphorylates phosphatidylinositol (PI) to generate PI3P....
ORGANISM(S): Homo Sapiens (human) 
2021-12-21 | PXD023533 | JPOST Repository
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