For proteomics, samples were heated for 10 min at 95 degrees. After cooling down to room temperature, 4 uL of 500 mM IAA in 5%SDS was added to a final concentration of 40 mM and incubated in the dark for 30 min. Undissolved matter was centrifuged for 8 min at 13,000 x g. The supernatant was saved an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
