ROME BioID2 raw proteomics data from ROME KO manuscript

ABSTRACT: For proteomics, samples were heated for 10 min at 95 degrees. After cooling down to room temperature, 4 uL of 500 mM IAA in 5%SDS was added to a final concentration of 40 mM and incubated in the dark for 30 min. Undissolved matter was centrifuged for 8 min at 13,000 x g. The supernatant was saved and used for downstream processing using a S-Trap column (ProtiFi, LLC). Proteins were digested with sequencing-grade Lys-C/trypsin (Promega) by incubation at 37 degrees overnight. Peptides were analyzed with a nanoAcquity UPLC system coupled with Orbitrap Fusion Lumos mass spectrometer (ThermoFisher) as previously described.

INSTRUMENT(S): Orbitrap Fusion Lumos, nanoACQUITY UPLC

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Aykut Uren  

PROVIDER: MSV000100665 | MassIVE | Fri Jan 30 10:01:00 GMT 2026

SECONDARY ACCESSION(S): PXD073850

REPOSITORIES: MassIVE