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1. Comparison of transcripts during growth in pure culture of a wild-type strain compared to a mutant deficient in the transcriptional activator encoded by the gene hrpG.

2. Transcriptome analysis for identification of the hrpG regulon by comparison of a rppG mutant to the wild type strain.
ORGANISM(S): Ralstonia solanacearum 
1. Comparison of transcripts during growth in pure culture of a wild-type strain compared to a mutant deficient in the transcriptional activator encoded by the gene prhG.

2. Transcriptome analysis for identification of the prhG regulon by comparison of a prhG overexpressing mutant to the wild...
ORGANISM(S): Ralstonia solanacearum 
Expressing the type III effector AWR5 of the phytopathogen Ralstonia solanacearum in yeast cells causes cessation of growth and transcriptional changes reminiscent of inhibition of the TOR pathway. Deletion of cdc55, a regulatory subunit of the PP2A Ser/Thr protein phosphatase eliminates the strong ...
ORGANISM(S): Saccharomyces cerevisiae 
The type III effector (T3E) AWR5 from the phytopathogen Ralstonia solanacearum causes disease in many plants relevant in agriculture. Heterologous expression in yeast of AWR5 from an integrated tetO promoter caused strong growth inhibition. In this series of experiments we investigate the possible c...
ORGANISM(S): Saccharomyces cerevisiae 
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