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TranscriptomicsEffect of loss of Gfi1b on hematopietic stem cells
Using Gfi1b conditional mice, deletion of gfi1b in the hematopietic system was induced by injecting MxCre tg Gfi1bfl/fl mice with pIpC. 30 days after injection, Cd150 pos, Cd 48 neg, Lin neg Sca and c-kit pos stem cells were sortrted from Gfi1bfl/fl and Mxcre tg Gfi1bfl/fl mice and analysed. We used...
ORGANISM(S): Mus musculus 
2010-09-03 | E-GEOD-20655 | biostudies-arrayexpress
TranscriptomicsMiz-1 is required to coordinate the pre-TCR-dependent transitional step in DN3/DN4 thymocytes
Lineage negative, CD44 negative, CD25 positive thymocytes were isolated from wt mice or Miz1 POZ-domain knockout mice to analyze the effect of loss of Miz1 in the DN3 population of T-cells We used the mouse Affymetrix MOE430-2 microarray to characterize global gene expression changes of DN3 thymocy...
ORGANISM(S): Mus musculus 
2011-08-23 | E-GEOD-28342 | biostudies-arrayexpress
TranscriptomicsGrowth factor independence 1 (Gfi1) regulates cell-fate decision of the bipotential granulocytic-monocytic precursor defined by the expression of CD48 as a new marker
Using bone marrow cells of GFP:Gfi1 knock in mice, we separated Gfi1-high and Gfi1-low expressing cells in the classical CD11b+, GR1-low monocytic cell fraction. We sorted CD11b+, GR1-low GFP:Gfi1-high and low cells as well as CD11b+, GR1-high granulocytes and CD11b-high, GR1-intermediate cells from...
ORGANISM(S): Mus musculus 
2012-12-19 | E-GEOD-35970 | biostudies-arrayexpress
TranscriptomicsDexamethasone responses in BRC-Abl (p210) transformed murine B-cells
For generation of pre-B cell lines, bone marrow from tibias and femurs of 4 to 8 week old C56Bl/6 mice (wt and vav-Gfi1b transgene) was infected with retroviral stocks of different BCR-Abl constructs (MSCV-GFP-IRESp210 or pSRa-p210-tkneo) and plated at a density of 5E+06 per 6 cm dish. All pre-B cel...
ORGANISM(S): Mus musculus 
2006-02-25 | E-GEOD-2342 | biostudies-arrayexpress
GenomicsThe Growth factor independence 1 (GFI1) 36N variant predisposes to Acute Myeloid Leukemia (AML) by inducing epigenetic changes at the Hoxa9 locus
GFI136N is a coding Single Nucleotide Polymorphism (SNP) in the gene GFI1 that increases the risk for Acute myeloid leukemia (AML) by 60%. It is present in 3-5% of Caucasians and has a prevalence of 12% among AML patients. We generated knockin mice expressing either the human GFI136N variant or the ...
ORGANISM(S): Mus musculus 
2012-12-04 | E-GEOD-31657 | biostudies-arrayexpress
TranscriptomicsExpression data from bone marrow derived granulocyte monocyte precursors (GMPs) from wt -, Gfi1 knock-out -, human-Gfi1-SNP36N knock in and human wt-Gfi1 knock in mice
The incidence of cancer is not only influenced by exposure to environmental factors such as toxic substances and chemotherapy, but is also a result of individual predispositions. Single Nucleotide Polymorphisms (SNPs) represent one such individual predisposition to cancer1. Gfi136N is a SNP in the c...
ORGANISM(S): Mus musculus 
2012-11-22 | E-GEOD-25551 | biostudies-arrayexpress
TranscriptomicsGrowth factor independence 1 (Gfi1) as a new target for leukemia therapy
Using (conditional) Gfi1 knock-out mice we show that ablation of the transcriptional repressor Gfi1 cures mice from lymphoid leukemia and reduces the expansion of primary human T-ALL xenografts in mice. We find that Gfi1 alters the p53 dependent transcriptional activation of a substantial subset of ...
ORGANISM(S): Mus musculus 
2012-10-03 | E-GEOD-32910 | biostudies-arrayexpress
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