HPKs from 3-5 healthy individual donors were pre-stimulated with TGFb (10ng/ml) plus IL-4 (20ng/ml) to upregulated the IL-9R (Das et al., 2019). Next day, HPKs were stimulated with IL-9 (50ng/ml) or in combination with IFNg (10ng/ml) for 24 hours at 37 degree C with 5% CO2. Sample preparation for L...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
