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ProteomicsAn Immunological Synapse Formation Between T Regulatory Cells and Cancer-Associated Fibroblasts Promotes Tumour Development
The cells were lysed in a buffer consisting of 4% SDS, 0.1 M DTT, 100 mM Tris/HCl; pH 7.6 and heated for 5 minutes at 99℃. The protein extracts were processed according to theSp3 protocol. The reduced cysteine residues were alkylated in 200 mM iodoacetamide (Acros Organics). 20 μg of beads (1:1 mixt...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-08 | PXD047024 | Pride
Autophagy Cancer-associated fibroblasts Immune synapse Immunity T regulatory cells Anti-tumour immunity Antigen presentation 
ProteomicsProteomic analysis of bone marrow supernatants.
Sample preparation for proteomics analysis: Bone marrow supernatants (0.5 mL per sample) were concentrated with 3 kDa MWCO Amicon Ultra Centrifugal filter devices (Merck Millipore) up to a final volume of 30 uL. Protease inhibitors were added to the samples and the protein concentration was defined...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-08-07 | MSV000095541 | MassIVE
TranscriptomicsGene expression of murine iDCs isolated from tolerized MOG35-55-infused/MOG35-55-immunized or MOG35-55-immunized mice
Recent data from our group, demonstrate that infusion of myelin oligodendrocyte glycoprotein (MOG35-55) peptide, leads to induction of MOG35-55-specific Tregs and subsequent suppression of Experimental Autoimmune Encephalomyelitis (EAE), the mouse model of multiple sclerosis. Amelioration of EAE was...
ORGANISM(S): Mus musculus 
2015-04-20 | E-GEOD-47210 | biostudies-arrayexpress
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