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TranscriptomicsGene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals
The objective of the study was to analyze the impact of various lenght FSH withdrawal period (coasting) after the last FSH injection on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after 20, 44, 68 and 92 hours of coasting. For...
ORGANISM(S): Bos taurus 
2013-06-21 | E-GEOD-38345 | biostudies-arrayexpress
TranscriptomicsCellular and molecular characterization of the impact of laboratory setup on bovine in vitro embryo production
Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vitro in two different laboratories using the same protocol. Two different laboratories with same condition, site A blastocysts vs. Site B blastocysts, Biological replicates: 3 site A,...
ORGANISM(S): Bos taurus 
2012-03-31 | E-GEOD-34958 | biostudies-arrayexpress
TranscriptomicsTranscriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu
Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU Two kinds of 7 days post fertilization bovine embryos, in-vivo 7 days blastocysts vs. In vitro 7 days blastocysts, Biological replicates: 4 in-vitro, 4 in-viv...
ORGANISM(S): Bos taurus 
2012-03-31 | E-GEOD-35025 | biostudies-arrayexpress
TranscriptomicsDifferential Gene Expression Profile in Bovine Blastocysts Resulting from Hyperglycemia Exposure during Early Cleavage Stages
IVP blastocysts exposed to hyperglycaemia during early phase of development, i.e from zygote to 8-16 cells stages. Genes expression analysis is done at day 7.5 (7 days of embryo culture). Total RNA form glucose treated blastocysts (4 replicates of 10 embryos each) and control treated blastocysts (4 ...
ORGANISM(S): Bos taurus 
2011-10-19 | E-GEOD-33008 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of bovine oocytes at optimal coasting time combined with GnRH antagonist during the no-FSH period
The objective of the study was to analyze the effect of a GnRH antagonist used during the FSH withdrawal period (coasting) on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after a coasting duration of 68hrs (optimal condition, c...
ORGANISM(S): Bos taurus 
2014-02-25 | E-GEOD-44664 | biostudies-arrayexpress
TranscriptomicsDNA methylation analysis of bull semen according to peripubertal age (10 months vs. 16 months)
The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10, 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform. three condition experiment, 4 bulls, all collected ...
ORGANISM(S): Bos taurus 
2016-02-24 | E-GEOD-78231 | biostudies-arrayexpress
TranscriptomicsDNA methylation analysis of bull semen according to peripubertal age (12 months vs. 16 months)
The DNA methylation pattern in spermatozoa of bulls at different age was investigated. Spermatozoa collected from 4 different bulls at 10, 12 and 16 month each. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform. three condition experiment, 4 bulls, all collected ...
ORGANISM(S): Bos taurus 
2016-02-24 | E-GEOD-78232 | biostudies-arrayexpress
TranscriptomicsComprehensive across production systems assessment of the impact of in vitro micro-environment on the expression of messengers and long non-coding RNAs in the bovine blastocyst
In vitro production of cattle embryos over the past two decades has revealed several negative impacts, which have been attributed to the artificial microenvironment. Studies of embryos produced in vitro clearly point to aberrant gene expression levels. So far, the causal association between phenotyp...
ORGANISM(S): Bos taurus 
2011-03-11 | E-GEOD-27872 | biostudies-arrayexpress
TranscriptomicsComparison of Holstein and Jersey day 6 embryos
Some embryos display better survival potential to cryopreservation than others. The cause of such phenotype is still unclear and might be due to cell damage during cryopreservation, resulting from over-accumulation and composition of lipids. In cattle embryos, in vitro culture conditions have been s...
ORGANISM(S): Bos taurus 
2014-10-22 | E-GEOD-62553 | biostudies-arrayexpress
TranscriptomicsComparison of L-carnitine treated day 6 in vivo bovine embryos to controls, in both the Jersey and Holstein breeds.
The decreased rate of pregnancy obtained in cattle using frozen in vitro embryos compared to in vivo embryos has been associated with over-accumulation of intracellular lipid, which causes cell damage during cryopreservation. It is believed that the higher lipid content of blastomeres of bovine embr...
ORGANISM(S): Bos taurus 
2014-10-23 | E-GEOD-62595 | biostudies-arrayexpress
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