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ProteomicsAssessment of host hemoglobin accumulation in P. berghei heme pathway ferrochelatase knockout parasite food vacuole by iTRAQ
Two independent sets of fourplex iTRAQ labelling were carried out to assess the accumulation of host Hb in PbFCKO FVs. The results obtained have suggested that there is a ~3-4 -fold increase in Hb and chains in FCKO FVs as quantified by iTRAQ.
ORGANISM(S): Plasmodium Plasmodium berghei ANKA 
2022-05-10 | PXD031738 | Pride
Knockout (ko) Food vacuole (fv) Hemoglobin (hb) Wildtype (wt) Plasmodium berghei (pb) Ferrochelatase (fc) 
ProteomicsFood vacuole proteome of P. berghei heme pathway ferrochelatase knockout parasite by LC-MS/MS
LC-MS/MS of in-solution trypsin digested FV protein extracts was carried out for the FVs isolated from PbWT and PbFCKO parasites. The FV preparations had signature FV proteins such as plasmepsin IV, berghepain, aminopeptidases, subunits of vacuolar-type H+ATPase (V-type H ATPase), together with para...
ORGANISM(S): Plasmodium Plasmodium berghei ANKA 
2022-05-10 | PXD031736 | Pride
Knockout (ko) Food vacuole (fv) Wildtype (wt) Plasmodium berghei (pb) Ferrochelatase (fc) 
ProteomicsProteomic analysis of L-Methionine Sulfoximine (MSO)-treated Plasmodium falciparum by LC-MS/MS
LC-MS/MS of in-solution trypsin digested parasite protein extracts was carried out to examine the proteome of MSO-treated Pf parasites. Proteins were extracted from the untreated and MSO-treated parasite pellets of two independent experiments (Exp1: T7_R Vs T8; Exp2: T3_R Vs T4_R). For each experime...
ORGANISM(S): Plasmodium falciparum 58.1 
2023-05-27 | PXD032797 | Pride
Control Plasmodium falciparum (pf) Methionine sulfoximine (mso) Mso-treated 
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S-EPMC10215008 | biostudies-literature
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UnknownDistinct Molecular Processes Mediate Donor-derived Cell-free DNA Release From Kidney Transplants in Different Disease States.
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UnknownRobust estimation of bacterial cell count from optical density.
Not available
S-EPMC7499192 | biostudies-literature
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