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TranscriptomicsRNA-seq of seven stages of soybean cotyledon development
To understand the genetic mechanisms involved in the functional transition of cotyledons from non-photosynthetic storage tissue to metabolically active photosynthetic tissue during soybean seedling development, we constructed seven different RNA-Seq libraries using cotyledons from each developmental...
ORGANISM(S): Glycine max 
2014-07-29 | E-GEOD-42550 | biostudies-arrayexpress
TranscriptomicsFlux of transcript patterns during soybean seed development
Background: To understand gene expression networks leading to functional properties of the soybean seed, we have undertaken a detailed examination of soybean seed development during the stages of major accumulation of oils, proteins, and starches, as well as the desiccating and mature stages, using ...
ORGANISM(S): Glycine max 
2010-05-05 | E-GEOD-18620 | biostudies-arrayexpress
GenomicsGenome-wide identification of binding sites for NAC and YABBY transcription factors and co-regulated genes during soybean seedling development by ChIP-Seq and RNA-Seq.
In order to dissect the gene regulatory network during the functional transition of cotyledons from non-photosynthetic storage tissue to metabolically active photosynthetic tissue, we constructed ChIP-Seq libraries for NAC and YABBY transcription factors using pooled cotyledons from seedling develop...
ORGANISM(S): Glycine max 
2013-08-14 | E-GEOD-42422 | biostudies-arrayexpress
TranscriptomicsSomatic embryogenesis
Examination of cotyledons after 7, 14, 21 and 28 days on 40mg/L 2,4-D.
ORGANISM(S): Glycine max 
2010-06-05 | E-GEOD-136 | biostudies-arrayexpress
TranscriptomicsIdentification of soybean seed developmental stage specific and tissue specific miRNA targets by degradome sequencing .
Five degradome libraries were constructed from three different seed developmental stages. Separate degradome libraries were constructed for seed coat and cotyledons to identify the tissue specific miRNAs and their potential targets. Sequencing and analysis of degradome libraries gives identification...
ORGANISM(S): Glycine max 
2012-08-07 | E-GEOD-34433 | biostudies-arrayexpress
TranscriptomicsUsing RNA-Seq to Profile Soybean Seed Development from Fertilization to Maturity
To understand gene expression networks leading to functional properties and compositional traits of the soybean seed, we have undertaken a detailed examination of soybean seed development from a few days post-fertilization to the mature seed using Illumina high-throughput transcriptome sequencing (R...
ORGANISM(S): Glycine max 
2013-03-18 | E-GEOD-42871 | biostudies-arrayexpress
TranscriptomicsTissue specific expression of chalcone synthase siRNAs
In a previous study, seed coat and cotyledon tissues of Williams, Richland and T157 soybean lines were investigated to show tissue specificity of CHS siRNA expression (Tuteja et al., 2009). Here, we investigated more tissues such as leaf, root and germinating cotyledon to ascertain the tissue specif...
ORGANISM(S): Glycine max 
2014-02-13 | E-GEOD-49708 | biostudies-arrayexpress
TranscriptomicsRNA-Seq Profiling of a Defective Seed Coat Mutation in Glycine max Reveals Differential Expression of Proline Rich and Other Cell Wall Protein Transcripts
The plant cell wall performs a number of essential functions including providing shape to many different cell types and serving as a defense against potential pathogens. The net pattern mutation creates breaks in the seed coat of soybean (Glycine max) because of ruptured cell walls. Using RNA-Seq, w...
ORGANISM(S): Glycine max 
2014-05-16 | E-GEOD-54903 | biostudies-arrayexpress
TranscriptomicsTranscript profiling reveals expression differences in wild-type and glabrous soybean lines
Two high throughput transcript sequencing methods, Digital Gene Expression (DGE) Tag Profiling and RNA-Seq, were used to compare the transcriptional profiles in wild-type (cv. Clark standard, CS) and a mutant (cv. Clark glabrous, i.e., trichomeless or hairless, CG) soybean isoline that carries the d...
ORGANISM(S): Glycine max 
2011-10-25 | E-GEOD-33155 | biostudies-arrayexpress
GenomicsGlycine max
Glycine max Transcriptome or Gene expression
PRJNA84523 | ENA
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