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To identify mediators of obesity-linked reductions in PGC-1, we tested the effects of cellular nutrients in C2C12 myotubes. While overnight exposure to high insulin, glucose, glucosamine, or amino acids had no effect, saturated fatty acids (FA) potently reduced PGC-1a and b mRNA expression. Exper...
ORGANISM(S): Mus musculus 
Here we present a strategy for the identification and quantitation of histone methylation that utilizes nondeuterated acetic anhydride to fully acetylate all free lysine residues prior to digestion with trypsin and analysis by LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
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