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ProteomicsIdentification of the Kinase phosphorylation sites on AtGPA1 in presence of GDP
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., 2018, J. Biol. Chem 293(13): 4752-4766). Among them, we here cho...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2019-06-12 | MSV000083963 | MassIVE
ProteomicsIdentification of the Kinase phosphorylation sites on AtGPA1 in presence of GTP
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., J Biol Chem 293(13): 4752-4766). Among them, we choose 12 kinase...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2019-06-12 | MSV000083964 | MassIVE
ProteomicsSingle-cell proteomics differentiates Arabidopsis root cell types
Here we explore the feasibility of single-cell proteomics on plant samples. We focus on Arabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-04-17 | MSV000094558 | MassIVE
ProteomicsMYC2 phosphorylation
Mass spectrometry identification of MYC2 phosphorylation by the kinase Feronia
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2017-04-20 | MSV000080972 | MassIVE
ProteomicsUbiquitylome analysis of Arabidopsis roots.
Ubiquitylome analysis of Arabidopsis roots. 10 day old Arabidopsis roots were treated with mock, DMSO, or bortezomib for 3 hours. Root tissue was processed using the phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Ubiquitin remnant peptides were enriched using an anti-diglyci...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000086156 | MassIVE
ProteomicsArabidopsis thaliana whole-proteome BIN2 kinase assay
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
ProteomicsBIN2 and RAPTOR1B mutants phosphoproteomic analysis
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
ProteomicsProtein abundance and phosphorylation changes in feronia mutant arabidopsis rosette leaves
The goal of this study was to determine protein abundance and phosphorylation changes in the FERONIA mutant (fer) in Arabidopsis leaves. Seeds were germinated on 1/2MS with constant light (24hr light) for 10 days. Then, plants were transferred to soil and grown under short day condition (8-hour ligh...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-01-14 | MSV000084804 | MassIVE
Proteomics,MultiomicsDSK2 Phosphorylation
We used mass spectrometry to identify sites of DSK2 phosphorylation by BIN2.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-04-08 | MSV000079641 | MassIVE
ProteomicsPhosphorylation dynamics in a flg22-induced, G-protein dependent network reveals the RGS1 phosphatase
The Microbe Associated Molecular Pattern flg22 is recognized in a FLAGELLIN-SENSITVE 2-dependent manner in root tip cells. Here, we show a rapid and massive change in protein abundance and phosphorylation state of the Arabidopsis root cell proteome in wildtype and a mutant deficient in heterotrimer...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-08-16 | MSV000092672 | MassIVE
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