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Transcriptional profile comparison among PDIM positive and negative isolates. Standard in vitro exponential phase cultures of M. tuberculosis H37Rv ATCC27294 were plated on 7H11 plates. Individual colonies were sub-cultured in 7H9 broth and metabolically labeled with [14C] propionic acid. The apolar...
ORGANISM(S): Mycobacterium tuberculosis 
Genomic comparisons among PDIM positive and negative isolates. Standard in vitro exponential phase cultures of M. tuberculosis H37Rv ATCC27294 were plated on 7H11 plates. Individual colonies were sub-cultured in 7H9 broth and metabolically labeled with [14C] propionic acid. The apolar lipid fraction...
ORGANISM(S): Mycobacterium tuberculosis 
Cells were grown aerobically at 37 degC in Spizizen minimal medium either supplemented with 1 mM glycine betaine, 1.2 M NaCl or 1.2 M NaCl and 1 mM glycine betaine. Cells were harvested in the exponential growth phase at an OD600nm of 1.0. Proteome analysis was performed with samples from three biol...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-01-08 | MSV000084766 | MassIVE
The cell wall is essential for viability of fungi and is an effective drug target in pathogens such as Candida albicans. The contribution of posttranscriptional gene regulators to cell wall integrity in C. albicans is unknown. We show that the C. albicans Ccr4-Pop2 mRNA deadenylase, a regulator of m...
ORGANISM(S): Candida albicans 
T. pseudonana tiling arrays were used to validate gene models and to predict new genes in the genome of this diatom. The tiling array data validated transcription of about 41% (4,653) of the 11,390 computationally predicted genes. An additional 1,132 transcripts were identified that did not correspo...
ORGANISM(S): Thalassiosira pseudonana CCMP1335 
Wall teichoic acids (WTAs) are phosphate-rich, sugar-based polymers attached to the cell walls of most Gram-positive bacteria. In Staphylococcus aureus, these anionic polymers regulate cell division, protect cells from osmotic stress, mediate host colonization, and mask enzymatically susceptible pep...
ORGANISM(S): Staphylococcus aureus 
Identification of microRNA expressed in Arabidopsis plants grown at different ambient CO2 concentrations and different ambient temperatures Small RNA sequencing of Arabidopsis wild type ecotype Columbia (Col-0) rossette leaves at bolting stage grown at 430 M-BM-1 50ppm and 810 M-BM-1 50ppm CO2 conce...
ORGANISM(S): Arabidopsis thaliana 
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