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ProteomicsSpecific features and assembly of the plant mitochondrial complex I revealed by cryo-EM
Mitochondria are the powerhouses of eukaryotic cells and the site of essential metabolic reactions. Their main purpose is to maintain the high ATP/ADP ratio that is required to fuel the countless biochemical reactions taking place in eukaryotic cells1. This high ATP/ADP ratio is maintained through o...
ORGANISM(S): Brassica oleracea var. botrytis (Cauliflower) 
2020-10-26 | PXD017847 | Pride
Cryo-em Shotgun proteomics Complex i Mitochondria Plant 
ProteomicsHow to make a ribosome from mixed-up ribosomal RNA fragments in Chlamydomonas reinhardtii mitochondria
Mitochondria are essential components of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few, although essential, mitochondrial messenger RNAs still encoded by mitochondrial genomes. H...
ORGANISM(S): Chlamydomonas reinhardtii 
2022-03-15 | PXD024708 | Pride
Mitochondria   mitochondrial ribosome   mitoribosome   chlamydomonas reinhardtii   cryo-em   cryo-et   fragmented ribosomal rnas 
ProteomicsStructure of the full kinetoplastids mitoribosome and insight on its large subunit maturation
Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis1. They possess a single large mitochondrion, essential for the parasite survival2. In kinetoplastids mitochondrion, most of the molecular machineries and g...
ORGANISM(S): Leishmania tarentolae Trypanosoma cruzi cruzi 
2021-04-29 | PXD018981 | Pride
Leishmania Kinetoplastids Trypanosoma Mitochondria Ribosome Mitoribosome 
ProteomicsIdentification of Arabidopsis mitochondrial ribosome proteome
In order to identify the comprehensive composition of Arabidopsis mitochondrial ribosome proteins, a strategy based on complementary approaches was used. Classical biochemical purification of ribosomes was combined with the immunoprecipitation of mitoribosomes using a specific Arabidopsis mitoriboso...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-12-14 | PXD010324 | Pride
Arabidopsis Ribosome Mitochondria Nanolc-esi-ms/ms 
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