We conducted data-independent acquisition (DIA) proteomic profiling using a high-resolution Orbitrap Astral mass spectrometer on urine samples from 10 MG patients and 10 healthy volunteers.
HOIP truncations proteins GST-PUB, GST-NZF, GST-UBA and GST-RBR-LDD were purified, GST was used as a negative control. The tissue lysates from the C57BL/6 mice were incubated with glutathione-S-transferase (GST) beads which bound to the GST-HOIP truncation proteins beforehand. The pull-down componen...
