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ProteomicsHuman liver cell line LO2 LC-MS/MS
A hyperlipidaemia model of L02 cells was established using free fatty acids (FFA, oleate and palmitate, 2:1; Sigma-Aldrich, USA) at a final concentration of 1 mM for 24 h. Cells were allowed to attach overnight prior to HTG-medicated serum for 24 h and then exposed to FFA during drug treatment. Afte...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD045069 | Pride
Human Liver cell Lc-ms/ms 
ProteomicsIdentification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.
GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The...
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD050141 | Pride
Keratin  krt32 
ProteomicsDysregulation of N-glycosylation by RPN1 knockout in spermatocytes induces male infertility via impaired mitochondrial function in mice
N-glycosylation protein modifications play a crucial regulatory role in numerous biological processes, although their contribution to male reproduction in mammals remains largely undefined. Here, we found that Ribophorin I (RPN1), a subunit of oligosaccharyltransferase complex, is indispensable for ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD051379 | Pride
Rpn1  n-glycosylation  meiosis  spermatogenesis  fertility 
ProteomicsA Co-culture System Reveals a Cross-talk between Alveolar Epithelium and Pulmonary Microvascular Endothelium during SARS-CoV-2 Infection
SARS-CoV-2 seriously injures human alveoli and causes severe respiratory illness. Histopathologic evidences suggested alveolar-capillary barrier integrity is compromised in COVID-19 deaths, however, little is known about how it is disrupted. In this study, we investigated the effects of SARS-CoV-2 i...
ORGANISM(S): Homo sapiens (Human) 
2020-12-04 | PXD020470 | Pride
Hpaepic cells Sars-cov-2 Alveolar epithelium/endothelium co-culture system Hulec-5a cells 
UnknownProteomic and Phosphoproteomic Insights into a Signaling Hub Role for Cdc14 in Asexual Development and Multiple Stress Responses in Beauveria bassiana.
Not available
S-EPMC4824431 | biostudies-literature
UnknownMapping the N-linked glycosites of rice (Oryza sativa L.) germinating embryos.
Not available
S-EPMC5362090 | biostudies-literature
UnknownHistone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.
Not available
S-EPMC7857430 | biostudies-literature
UnknownQuantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer.
Not available
S-EPMC5292683 | biostudies-literature
TranscriptomicsTranslatome of A549, H1299 and HBE cells
We performed translatome and transcriptome sequencing of A549, H1299 and HBE cells and analyzed the translation ratio (TR) of all genes Total RNA and ribosome-bound RNA were extracted from A549, H1299 and HBE cells, and the polyA+ mRNA was sequenced using Illumina GAIIx and HiSeq-2000. The reads wer...
ORGANISM(S): Homo sapiens 
2013-03-05 | E-GEOD-42006 | biostudies-arrayexpress
UnknownComparative Proteomic Analysis of Lysine Acetylation in Fish CIK Cells Infected with Aquareovirus.
Not available
S-EPMC5713387 | biostudies-literature
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