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ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083957 | MassIVE
TranscriptomicsTranscriptomics analysis of gene expression in normal and METTL3 or WTAP deficient Human 293T cells
RNA was isolated from and METTL3M-oM-
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50582 | biostudies-arrayexpress
TranscriptomicsBinding motif analysis of METTL3，WTAP in METTL3，WTAP overexpressed Human 293T cells
label the cells overexpressed Myc tagged METTL3 and Flag tagged WTAP with 4-SU, the RNA bound by METT3,WTAP can be got by Myc or Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library preparation using a TruSeq™ RN...
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50580 | biostudies-arrayexpress
TranscriptomicsTranscriptomics analysis of gene expression in normal and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 or SRSF10 deficient human HeLa cells
RNA was isolated from and YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 deficient human HeLa cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) o...
ORGANISM(S): Homo sapiens 
2016-02-17 | E-GEOD-71095 | biostudies-arrayexpress
TranscriptomicsPAR-CLIP analysis of YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 in human HeLa cells
Label the cells overexpressed Flag tagged YTHDC1, SRSF1, SRSF3, SRSF7, SRSF9 and SRSF10 with 4-SU, the RNA bound by YTHDC1 and SRSF proteins can be got by Flag IP followed by RNA isolation by using the TRIzol (Invitrogen) reagent by following the company manual.the RNA was then used for library prep...
ORGANISM(S): Homo sapiens 
2016-02-17 | E-GEOD-71096 | biostudies-arrayexpress
TranscriptomicsMETTL3 and WTAP
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2014-01-16 | E-GEOD-50583 | biostudies-arrayexpress
TranscriptomicsThe contribution of Alu exons to the human proteome
Alu element is a major contributor to lineage-specific new exons in the primate and human genomes. Recent studies indicate that some Alu exons have high transcript inclusion levels or tissue-specific splicing profiles, and may play important regulatory roles in modulating mRNA degradation or transla...
ORGANISM(S): Homo sapiens 
2015-12-10 | E-GEOD-65999 | biostudies-arrayexpress
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