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ProteomicsCharacterization of SPSB1-regulated proteins following CRISPR APA engineering
Proteomics analysis was performed in 22Rv1, LNCaP-abl and BPH1 cells after transfection of 3'UTRCES-SPSB1 systems
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD071209 | Pride
Prostate cancer Crispr Alternative polyadenylation 
GenomicsLigand-dependent genomic function of glucocorticoid receptor in triple-negative breast cancer
Glucocorticoids (GC) have been widely used as coadjuvants in the treatment of solid tumors, but GC treatment may be associated with poor pharmacotherapeutic response and/or prognosis. The genomic action of GC in these tumors is largely unknown. Here we find that dexamethasone (Dex, a synthetic GC) r...
ORGANISM(S): Homo sapiens 
2015-09-18 | E-GEOD-56022 | biostudies-arrayexpress
ProteomicsEstablishment of a novel in vitro transcription assay
RNA Polymerase II (Pol II) transcriptional recycling is an underappreciated mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptio...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD027143 | Pride
Lc-ms/ms Transcription 
ProteomicsEstablishment of a novel in vitro transcription assay
RNA Polymerase II (Pol II) transcriptional recycling is an underappreciated mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptio...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD017881 | Pride
In vitro transcription Lc-ms/ms 
ProteomicsProteomic analysis of SPSB1 interactome
Flag-tagged SPSB1 was overexpressed in human 293FT cells followed by immunoprecipitation using ANTI-FLAG M2-Agarose Affinity Gel. The enriched SPSB1-interacting proteins on affinity gel were subject to quantitative proteomics analysis.
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD057059 | Pride
GenomicsNext Generation Sequencing Facilitates Quantitative Analysis of effect of knockdown of GATA2 on AR binding sites
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare AR binding activity in LNCaP cells with and without knockdown of GATA2. Methods: LNCaP cells between passage number 32-34 were used for assay. Cells are tr...
ORGANISM(S): Homo sapiens 
2014-01-21 | E-GEOD-52725 | biostudies-arrayexpress
GenomicsRedefinition of Human Androgen Responsive Elements [FOX_ChIP_exo]
The androgen receptor (AR) mediates the action of androgens by binding to androgen-responsive elements (AREs) and subsequently regulating target genes involved in prostate carcinogenesis. The precise locations, true nature, and functional roles of AREs in human prostate cancer are still unknown. Her...
ORGANISM(S): Homo sapiens 
2015-01-01 | E-GEOD-43678 | biostudies-arrayexpress
GenomicsRedefinition of Human Androgen Responsive Elements [MNase-Seq, ChIP_seq]
The androgen receptor (AR) mediates the action of androgens by binding to androgen-responsive elements (AREs) and subsequently regulating target genes involved in prostate carcinogenesis. The precise locations, true nature, and functional roles of AREs in human prostate cancer are still unknown. Her...
ORGANISM(S): Homo sapiens 
2015-01-01 | E-GEOD-43720 | biostudies-arrayexpress
GenomicsIntegrative analysis identifies targetable CREB1/FoxA1 transcriptional co-regulation as a predictor of prostate cancer recurrence
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this work is comprehensive analysis of target genes co-regulated by CREB1 and FoxA1 in prostate cancer cell models, tissues, and circulating tumor cells (CTCs). Expression of CREB1/...
ORGANISM(S): Homo sapiens 
2016-01-13 | E-GEOD-63034 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human LNCaP cell line
Androgen receptor (AR) is a ligand-dependent transcription factor that plays a key role in the onset and progression of prostate cancer. Surprisingly little is known of AR binding sites and collaborating transcription factors in the human genome. Here we have identified the DNA sequence motifs that ...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7868 | biostudies-arrayexpress
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