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Y. pestis strain 201 and mutant ΔyscI cultures grown under Mh (37°C, TMH with no calcium) condition were used as the experimental and control groups, respectively. After 8 h of incubation, the cultures supernatants were collected by centfugation and concentrated using centrifugal filters. The concen...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD020060 |
Y. pestis strain 201 cultures grown under Mh (37°C, TMH with no calcium) and Fv (26°C, TMH with 2.5M calcium) conditions were used as the experimental and control groups, respectively. After 8 h of incubation, the cultures supernatants were collected by centfugation and concentrated using centrifuga...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD020062 |
The secreted proteome and bacteria proteome of yersinia pestis at 37℃ mimic human. Y. pestis strain 201 cultures grown under Mh (37°C, TMH with no calcium) condition were used as the experimental and control groups, respectively. After 8 h of incubation, the cultures supernatants were collected by c...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD023183 |
Y. pestis strain 201 and mutant ΔyscI cultures grown under Mh (37°C, TMH with no calcium) condition were used as the experimental and control groups, respectively. After 8 h of incubation, the bacterial pellets were collected by centfugation, re-suspended in lysis buffer (8 M urea in PBS, pH 7.4) an...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD023156 |
The secreted proteome and bacteria proteome of yersinia pestis at 26 ℃ mimic flea. Y. pestis strain 201 cultures grown under Fv (26°C, TMH with 2.5M calcium) condition. The bacteria pellets and supernant were used as the experimental and control groups, respectively. After 8 h of incubation, the cul...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD023157 |
Y. pestis strain 201 cultures grown under Mh (37°C, TMH with no calcium) and Fv (26°C, TMH with 2.5M calcium) conditions were used as the experimental and control groups, respectively. After 8 h of incubation, the bacterial pellets were collected by centfugation, re-suspended in lysis buffer (8 M ur...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD020063 |
Parallel reaction monitoring (PRM) analysis was used to confirm changes in abundance of the target proteins (AceF, SodA, YopD, YopE, GroEL, TerE, and OsmY2),. Equal amounts of proteins from the culture supernatants and cell pellets were separated by SDS-PAGE, and bands corresponding to each of the t...
ORGANISM(S): Yersinia Pestis Co92 
2020-12-16 | PXD020061 |
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