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ProteomicsArabidopsis meiotic-specific recombinase DMC1 IP-MS
To search for regulators of DMC1 stability, we generated DMC1-FLAG transgenic Arabidopsis plants and conducted immunoprecipitation-mass spectrometry (IP-MS) assay. 6,477 peptides corresponding to 2309 proteins were identified and 1009 proteins remained after removing background interactions using pl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-24 | PXD038126 | Pride
Dmc1 Arabidopsis Lc-ms/ms 
ProteomicsArabidopsis DMC1 ubiquitination sites identification, LC-MS/MS
To characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. col...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-24 | PXD038116 | Pride
Dmc1 Arabidopsis Lc-ms/ms 
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