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ProteomicsArabidopsis DMC1 ubiquitination sites identification, LC-MS/MS
To characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. col...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-24 | PXD038116 | Pride
Dmc1 Arabidopsis Lc-ms/ms 
TranscriptomicsssDNA mapping in dmc1 strains
ssDNA enrichment was used to map and compare DSB hotspots in dmc1, pch2 dmc1, sir2 dmc1, orc1-161 dmc1, dmc1 rdnadelete and dmc1 chr2:12 translocation strains. 6 samples, 2 replicates each. Averaged data is available as a supplementary file on the Series record (below).
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-14 | E-GEOD-30071 | biostudies-arrayexpress
ProteomicsArabidopsis meiotic-specific recombinase DMC1 IP-MS
To search for regulators of DMC1 stability, we generated DMC1-FLAG transgenic Arabidopsis plants and conducted immunoprecipitation-mass spectrometry (IP-MS) assay. 6,477 peptides corresponding to 2309 proteins were identified and 1009 proteins remained after removing background interactions using pl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-10-24 | PXD038126 | Pride
Dmc1 Arabidopsis Lc-ms/ms 
GenomicsGenome-wide profiling of the meiotic recombinase DMC1, the chromosome axis protein ASY1 and chromatin states in hexaploid wheat (ChIP-seq)
We performed ChIP-seq for the meiotic strand exchange protein DMC1, which marks an early stage in the meiotic recombination pathway, and the chromosome axis protein ASY1, which promotes interhomolog synapsis and recombination in plants, using tissue collected from immature pre-emergence spikes from ...
ORGANISM(S): Triticum aestivum 
2021-07-07 | E-MTAB-9632 | biostudies-arrayexpress
GenomicsssDNA mapping in dmc1 strains
ssDNA enrichment was used to map and compare DSB hotspots in dmc1, pch2 dmc1, sir2 dmc1, orc1-161 dmc1, dmc1 rdnadelete and dmc1 chr2:12 translocation strains.
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-15 | GSE30071 | GEO
TranscriptomicsMeiotic DNA double strand breaks in the yeast Saccaromyces cerevisiae
DNA double-strand breaks (DSBs) initiate meiotic recombination. Past DSB-mapping studies have used rad50S or sae2? mutants, which are defective in break processing, to accumulate DSBs, and report large (= 50 kb) “DSB-hot” regions that are separated by “DSB-cold” domains of similar size. Substantial ...
ORGANISM(S): Saccharomyces cerevisiae 
2007-12-30 | E-GEOD-8981 | biostudies-arrayexpress
GenomicsHumanized PRDM9 Mouse Testis H3K4me3 and DMC1 ChIP-seq
We report testis H3K4me3 and DMC1 enrichment at DSB hotspots in various mice to examine differences between infertile hybrid mice and mice that have been humanized at PRDM9, which have rescued fertility. We find that infertile mice have an excess of "asymmetric" DSB hotspots, where both H3K4me3 and ...
ORGANISM(S): Mus musculus 
2016-02-02 | GSE73833 | GEO
GenomicsDMC1 ChIP-seq from B6xCAST F1 PRDM9-Humanized/CAST testes
DMC1 ChIP-seq from B6xCAST F1 PRDM9-Humanized/CAST testes
PRJNA514744 | ENA
UnknownDMC1 attenuates RAD51-mediated recombination in Arabidopsis.
Not available
S-EPMC9451096 | biostudies-literature
GenomicsFIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in meiotic DNA replication and recombination
FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in meiotic DNA replication and recombination
PRJNA947545 | ENA
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