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TranscriptomicsSerum Starvation/Refeeding RNA vs. RAP
Low abundance mRNAs are more difficult to examine using microarrays than high abundance mRNAs due to the effect of concentration on hybridization kinetics, signal-to-noise ratios, and interference between signals from mRNA isoforms. Individual sequences in LCRs are more highly represented than in th...
ORGANISM(S): Homo sapiens 
2010-06-10 | E-GEOD-2655 | biostudies-arrayexpress
Transcriptomics267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Two prostate immortalized cell lines, 267B1, RWPE-1, Ml-csv40, and five prostate cancer cell lines, PC3, PC3M, PC3M-Pro4, PC3M-LN4 and LNCaP are included in this experiment. Their methylation profile are detected using human promoter array (v11). mRNA expression level are profiled with Affymetrix U1...
ORGANISM(S): Homo sapiens 
2010-06-09 | E-GEOD-951 | biostudies-arrayexpress
TranscriptomicsCC2_Navarre_14028srpoSlowhns-_IP_pHNStagged_antiHA
array analysis of immunoprecipitated DNA from tagged HNS proteins in 14028s mutant strain (rpoS low, hns minus) four slides, FLAG-tagged HNS versus gDNA and HA-tagged HNS versus gDNA IP'd using anti-HA, including dye-swaps
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2010-07-01 | E-GEOD-4881 | biostudies-arrayexpress
TranscriptomicsEgr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip
Previous studies have revealed that UV-stimulation of a variety of cells leads to activation of the EGF receptor, induction of Egr1, growth inhibition and apoptosis. On the other hand both Egr1 and EGF receptor activation are implicated in promoting the progression of prostate cancer. We treated M...
ORGANISM(S): Homo sapiens 
2010-05-06 | E-GEOD-10585 | biostudies-arrayexpress
TranscriptomicsMapping of hns sites in Typhimurium 14028 using a tiling oligo array
The NimbleGen array contains 387,000 46~50-mer oligos, including 377,230 salmonella probes whose sequences only hit genome once. The probes were designed based on Salmonella typhimurium LT2 (NC_003197) genome, with a moving window of about 12 bases. Raw intensities were normalized by the median inte...
ORGANISM(S): Salmonella 
2010-07-01 | E-GEOD-4879 | biostudies-arrayexpress
TranscriptomicsCHIP Analysis - c-Jun/ATF2
Identification of promoters bound by c-Jun/ATF2 during rapid large-scale gene activation following genotoxic stress
ORGANISM(S): Homo sapiens 
2010-06-05 | E-GEOD-1810 | biostudies-arrayexpress
TranscriptomicsTL2_14028s_invA::cam_IP_BALBc_mouse_LibS_invA_vs_Lib0_invA_Mice
Salmonella typhimurium 14028s invA::cam Transposon library recovered from spleen 2 days after IP passage of 2.2 x 10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis 6 biological repl...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2008-08-31 | E-GEOD-7059 | biostudies-arrayexpress
TranscriptomicsTL2_14028s_M9+arabinose_(3_passages)_LibM9_vs_Lib0_Exp.2
Salmonella typhimurium 14028s Transposon library recovered after three consecutive rounds of growth to late log phase in M9 minimal medium (arabinose 0.4%) at 37°C with aeration, compared to an expansion of the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywor...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2010-06-20 | E-GEOD-7058 | biostudies-arrayexpress
TranscriptomicsChromatin modifications induced by PML-RARalpha repress critical targets in leukemogenesis as analyzed by ChIP-Chip.
A global view of PML-RAR? transcriptional functions was obtained by genome-wide binding and chromatin modification analyses, combined with genome wide expression data. Complete Abstract: The translocation t(15;17) generates the chimeric PML-RAR? transcription factor that is the initiating event of ...
ORGANISM(S): Homo sapiens 
2008-09-06 | E-GEOD-12641 | biostudies-arrayexpress
ProteomicsProteomic research on effect of EIF4A1 inhibition in MYC-amplified Group 3 medulloblastoma
As the most life-threatening subtype of pediatric medulloblastoma (MB), MYC-amplified Group 3 (G3) MB lacks effective and selective therapeutics. We tried to indirectly target MYC (oncogenic driver and cancer-dependent molecule) production via inhibiting translational machinery. Through multiple dat...
ORGANISM(S): Homo Sapiens 
2020-10-15 | PXD022014 |
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