Proteomic analysis of hydrolase levels in the lysosomes from macrophages stimulated with or without LPS via liquid chromatography–mass spectrometry (LC-MS), highlighting the expected LPS-induced increase in accumulation of proteases, ribonucleases (RNase) and other hydrolases.
To investigate the degradation activity of proteases inside lysosomal "TRAP", we conducted proteomic analysis of lysosomal "TRAP" with trapped authentic SARS-CoV-2.
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2
To gain a deeper insight into the residual SARS-CoV-2 peptides after degradation, we directly detected fragments (lower than 10 (kilodalton) kDa) of degraded SARS-CoV-2 via LC-MS, and overlapped these with the 3D crystal structure of SARS-CoV-2 proteins.
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2
To investigate the degradation activity of proteases inside lysosomal "TRAP", we conducted proteomic analysis of lysosomal "TRAP" with trapped authentic SARS-CoV-2.
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2
The novel Coronavirus SARS-CoV-2 causes a respiratory illness called COVID-19 leading to a pandemic. An effective preventive vaccine against this virus is urgently needed.
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2
