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ProteomicsPTPRR interactome detection using PUP-IT (pupylation-based interaction tagging) Mass Spectrometry
To further stably express PTPRR (WT)-PafA or PTPRR (DA)-PafA in iPUP OVCAR5 cell, we subcloned PTPRR-WT or DA, respectively, into the PafA-IRES-EGFP plasmid. Each plasmid was packed into a lentivirus and then transduced into iPUP OVACR5 cells for 48 h. GFP-positive cells were sorted by flow cytometr...
ORGANISM(S): Homo sapiens (Human) 
2019-10-30 | PXD015352 | Pride
Lc-ms Ptprr 
ProteomicsFER-mediated phosphorylation and PIK3R2 recruitment on IRS4 promotes AKT activation and tumorigenesis in ovarian cancer
Tyrosine phosphorylation, orchestrated by tyrosine kinases and phosphatases, modulates a multi-layered signaling network in a time and space dependent manner. Dysregulation of this post-translational modification is inevitably associated with pathological diseases. Our previous work has demonstrated...
ORGANISM(S): Homo Sapiens 
2020-10-19 | PXD022084 |
ProteomicsTHEMIS is a novel substrate and allosteric activator of SHP1, playing dual roles during T cell development
By combining strategies of substrate trapping for PTPs and pupylation-based interaction tagging (PUP-IT) for protein-protein interaction, we develop a systematic proximity labeling methodology PEPSI in identifying novel substrate for PTPs. We apply this approach to SHP1 and surprisingly, identified ...
ORGANISM(S): Homo Sapiens 
2023-08-31 | PXD028689 |
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