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ProteomicsSIRT5 ameliorates cardiac fibrosis via PCK2 de-succinylation–mediated metabolic reprogramming in cardiac fibroblasts
WT and Sirt5-/- CFBs were stimulated with TGF-β1 (10 ng/mL) for 36 hours. Cells were harvested, snap-frozen, and stored at -80℃. The experiment was independently repeated six times at different time points. Cells from each replicate were pooled to yield more than 3 mg of protein per group. Pooled WT...
ORGANISM(S): Mus Musculus 
2025-11-15 | PXD070811 |
ProteomicsMi-2β promotes immune evasion in melanoma by activating EZH2 methylation
For identification of Mi-2β-interacting proteins, B16F10 cells (1 × 108) were collected and washed three times with PBS and lysed in lysis buffer on ice for 30 min. Cell lysates were collected and then isolated over night by anti-Mi-2β magnetic agarose beads. The anti-Mi-2β magnetic agarose beads we...
ORGANISM(S): Mus Musculus 
2024-02-18 | PXD049467 |
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