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Proteomics on Human breast cancer tissue samples. 9 patients with high risk or 9 patiens low risk MammaPrint breast cancer. Membran fraction. Proteins were reduced, alkylated, digested with Lys-C and Trypsin, and labelled with iTRAQ4plex: 114; high risk; 115; low risk.
ORGANISM(S): Homo sapiens (Human) 
2013-01-08 | PXD000066 | Pride
Specification to primordial germ cells (PGCs) occurs under the mesoderm induction signals during gastrulation. Here, we found that Akt activation in embryonic stem (ES) cells generated self-renewing spheres during mesodermal differentiation induction and that the differentiation status of the spher...
ORGANISM(S): Mus musculus 
Total RNA was isolated from soybean mesophyll protolplasts over-expressing GmDREB1B1 gene
ORGANISM(S): Glycine max 
Total RNA was isolated from 18-day-old seedlings of the transgenic Arabidopsis plants grown on GM agar plates with or without treatment of 1 uM dexamethazon
ORGANISM(S): Arabidopsis thaliana 
Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX col...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-15 | MSV000079583 | MassIVE
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