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TranscriptomicsTranscription profiling of S. pombe reveals Cyclin dependent kinase inhibits re-initiation of a normal S-phase program during G2
To achieve faithful replication of the genome once in each cell cycle, re-initiation of S-phase is prevented in G2 and origins are restricted from re-firing within S-phase. We have investigated the block to re-replication during G2 in fission yeast. The DNA synthesis that occurs when G2/M cyclin d...
ORGANISM(S): Schizosaccharomyces pombe 
2009-05-13 | E-MTAB-105 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast wild type and zinc regulatory null mutant grown in low zinc growth conditions
Transcription profile of low zinc growth condition in zinc regulatory null mutant
ORGANISM(S): Schizosaccharomyces pombe 
2008-02-22 | E-TABM-427 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of S. pombe co-overexpressing DNA synthesis initiation factors cdc18 (cdc6) and cdt1 to investigate gene copy number increases
During S-phase of the eukaryotic cell cycle, controls exist to ensure that replication origins fire only once and become inactivated after traverse of a replication fork. Failure of these controls results in local amplification of the genome which can lead to gene copy increases important for both ...
ORGANISM(S): Schizosaccharomyces pombe 
2010-08-13 | E-MTAB-139 | biostudies-arrayexpress
TranscriptomicsMyb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast
The TTAGGG motif is common to two seemingly unrelated dimensions of chromatin function M-^V the vertebrate telomere repeat and the promoter regions of many Schizosaccharomyces pombe genes, including all of those encoding canonical histones. The essential S. pombe protein Teb1 contains two Myb-like D...
ORGANISM(S): Schizosaccharomyces pombe 
2013-08-14 | E-MTAB-1253 | biostudies-arrayexpress
no omics definedA coordintate global control over cellular transcription
Although much is known about the regulation of eukaryotic transcription, the global controls which determine rates of total transcription within a cell are not well understood. We have investigated the effects that the DNA to protein ratio has on both total cellular transcription and the transcripti...
ORGANISM(S): Schizosaccharomyces pombe 
2011-09-23 | E-TABM-1075 | biostudies-arrayexpress
Transcriptomicsteb1 hypomorphic allele
The essential S. pombe protein Teb1 contains two Myb-like DNA binding domains related to those found in telomere proteins and binds the human telomere repeat sequence TTAGGG. Here we analysed the consequences of reduced Teb1 function. The hypomorphic allele, teb1-1, confers reduced levels of histone...
ORGANISM(S): Schizosaccharomyces pombe 
2012-08-31 | E-MTAB-1251 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast Pht1 (H2A.Z) deletion mutants
Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate that the fission yeast Swr1 1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or an unacetylatable mutation of Ph...
ORGANISM(S): Schizosaccharomyces pombe 
2010-11-05 | E-TABM-842 | biostudies-arrayexpress
TranscriptomicsTranslation profiling of Schizosaccharomyces pombe size separated polysome fractions provides data on average numbers of associated ribosomes for most transcripts
To obtain translational profiles for all mRNAs, polysome preparations are separated according to their size using a sucrose gradient and the mRNAs in each fraction are identified and quantified with DNA microarrays. Starting with exponentially growing cells, we analyzed 12 polysome fractions using D...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-222 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast timecourse mcs6 mutant
The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression w...
ORGANISM(S): Schizosaccharomyces pombe 
2004-11-01 | E-MEXP-192 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fission yeast timecourse pmh1 mutant
The fission yeast Mcs6-Mcs2-Pmh1 complex (homologous to metazoan Cdk7-cyclin HMat1) performs dual functions: in cell cycle control, as a CDK-activating kinase; and in transcription by RNA polymerase (Pol) II, as part of TFIIH. It is unknown whether that linkage serves to coordinate gene expression w...
ORGANISM(S): Schizosaccharomyces pombe 
2004-11-01 | E-MEXP-191 | biostudies-arrayexpress
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