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Genomics[E-MTAB-131] Estrogen Receptor alpha estrogen-responsive binding sites in MCF-7
We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer...
ORGANISM(S): Homo sapiens 
2011-02-05 | GSE25684 | GEO
TranscriptomicsGene expression profile in a breast cancer cell line upon hormonal treatments
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-alpha tagged with TAP-tag at the C-term (C-TAP-ER-alpha). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) fo...
ORGANISM(S): Homo sapiens 
2012-06-25 | E-MTAB-1196 | biostudies-arrayexpress
TranscriptomicsGene expression profile in breast cancer cell lines using miRNA over-expression
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag at the C-term (C-TAP-ER-beta). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) for ...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-MTAB-1493 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profile between ER-beta- and ER-beta+ breast tumors.
FFPE tissues samples were cut in 5-µm-thick sections on a microtome with a disposable blade. RNA was extracted either from three or eight sequential sections using miRNeasy FFPE Kit (QIAGEN), following the steps described in the manufacturers protocol. RNA extracted from same biological samples and...
ORGANISM(S): Homo sapiens 
2011-10-14 | E-TABM-1054 | biostudies-arrayexpress
GenomicsChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-MTAB-345 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human MCF-7 cells with ER-beta tagged with TAP-tag at the C-term or N-term or expressing ER-alpha tagged
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha). All were grown in Dulbecco's modified Eagle's medium (DMEM). Then c...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1052 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human MCF-7 breast cell clones expressing ER-beta tagged with TAP-tag at the C-terminal or N-terminal
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.Cells were cultured in standard growth condit...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1053 | biostudies-arrayexpress
no omics definedMicroRNA profiling by array of human MCF-7 breast cancer cells with ER-beta tagged at the C-terminal or N-terminal and treated with 17-beta-estradiol
Human estrogen-responsive breast cancer cell line MCF-7 TET Off (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.(...
ORGANISM(S): Homo sapiens 
2011-07-29 | E-TABM-1055 | biostudies-arrayexpress
GenomicsChIP-Seq of human MCF-7 cells with ER-alpha anitbody following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer...
ORGANISM(S): Homo sapiens 
2010-01-11 | E-MTAB-131 | biostudies-arrayexpress
TranscriptomicsTime-series miRNA breast cancer cells
Human estrogen-responsive breast cancer cell line MCF7 and ZR75.1 were treated with 10 nM of17beta-estradiol, or vehicle (ETOH). miRNA expression was analyzed on total RNA extracted before and after 6, 12, 24, and 72 hours of hormone stimulation. Total RNA was fluorescently labelled, amplified in tr...
ORGANISM(S): Homo sapiens 
2012-01-05 | E-TABM-1194 | biostudies-arrayexpress
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