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TranscriptomicsThe eIF2 kinase PERK and the integrated stress response facilitate activation of ATF6 during endoplasmic reticulum stress
Disruptions of the endoplasmic reticulum (ER) that perturb protein folding cause ER stress and elicit an unfolded protein response (UPR) that involves translational and transcriptional changes in gene expression aimed at expanding the ER processing capacity and alleviating cellular injury. Three ER ...
ORGANISM(S): Mus musculus 
2012-01-20 | E-GEOD-29929 | biostudies-arrayexpress
TranscriptomicsMicroarray to find CHOP dependent genes in response to proteasome inhibition
Environmental stresses that disrupt protein homeostasis induce phosphorylation of eIF2, triggering repression of global protein synthesis coincident with preferential translation of ATF4, a transcriptional activator of the Integrated stress response (ISR). Depending on the extent of protein disrupti...
ORGANISM(S): Mus musculus 
2013-09-05 | E-GEOD-43713 | biostudies-arrayexpress
TranscriptomicsThe Toxoplasma unfolded protein response
To characterize the nature of the unfolded protein response in this Toxoplasma, we carried out microarray analyses to measure the changes in the transcriptome and in translational control during ER stress. Total RNA or RNA fractionated into monosomes or polysomes were purified from parasites treated...
ORGANISM(S): Toxoplasma gondii 
2013-05-24 | E-GEOD-43722 | biostudies-arrayexpress
TranscriptomicsSelective control of metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae
When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. We examine how yeast growth and tRNA charging or aminoacylation is affected during amino acid depletion in the presence and absence of GCN2. tRNA charging is measured using ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-08-01 | E-GEOD-22452 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of yeast gcn2, gcn4 and gln3 deletion mutants treated with rapamycin or 3-amino-1,2,4-triazole (3-AT)
Two nutrient sensing and regulatory pathways, the general amino acid control (GAAC) and the target of rapamycin (TOR), control yeast growth and metabolism in response to changes in nutrient availability. Starvation for amino acids activates the GAAC pathway, involving Gcn2p phosphorylation of eIF2 ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-03-07 | E-GEOD-15254 | biostudies-arrayexpress
ProteomicsPERK protein kinase facilitates keratinocyte collective cell migration by engagement with cell adhesion molecules independent of its kinase activity
Proximity labeling experiments (BioID) were performed on Human NTERT keratinocytes depleted of PERK (EIF2AK3, PERK-KO) re-expressing a WT PERK containing a C-terminal UltraID tag. PERK-KO and PERKUltraID cells were treated with differentiation medium containing 50 uM of exogenous Biotin for 6 h, wa...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-11 | MSV000098126 | MassIVE
TranscriptomicsMicroarray to find ATF5 dependent genes in response to proteasome inhibition
Environmental stresses that disrupt protein homeostasis induce phosphorylation of eIF2, triggering repression of global protein synthesis coincident with preferential translation of ATF4, a transcriptional activator of the Integrated stress response (ISR). Depending on the extent of protein disrupti...
ORGANISM(S): Mus musculus 
2013-09-05 | E-GEOD-43714 | biostudies-arrayexpress
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