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ProteomicsDevelopment of a Mass Spectrometry-Based Quantitation Assay for Human Body Fluid Proteins using a Wheat Germ Cell-Free Synthesis System
In this study, we have developed a large-scale SRM assay targeting human body fluid proteins based on publicly available proteomics data. We first aimed to produce, in a high throughput manner, a peptide reference library. For this purpose, we selected 2,201 targeted peptides representing 1,215 prot...
ORGANISM(S): Homo sapiens (Human) 
2016-05-24 | PXD002090 | Pride
Wheat germ cell-free system Human body fluid proteins Targeted proteomics Reference peptide library 
UnknownWheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates.
Not available
S-EPMC2292889 | biostudies-literature
ProteomicsDe novo reconstitution and isolation of nucleosomes using the wheat germ cell-free protein synthesis
DNA is packaged with histones and non-histone proteins to form chromatin that impinges on all nuclear processes such as transcription, replication and repair. A complete molecular understanding of these processes requires analysis of chromatin context. In this study, we report a simple and robust sy...
ORGANISM(S): Drosophila Melanogaster 
PXD011113 | panorama
UnknownPlasmodium vivax: comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays.
Not available
S-EPMC3224337 | biostudies-literature
UnknownReconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system.
Not available
S-EPMC7708258 | biostudies-literature
UnknownProtein structural biology using cell-free platform from wheat germ.
Not available
S-EPMC6244559 | biostudies-literature
UnknownEasy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.
Not available
S-EPMC8027086 | biostudies-literature
UnknownDevelopment of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts.
Not available
S-EPMC2906421 | biostudies-literature
UnknownImmunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate.
Not available
S-EPMC5380959 | biostudies-literature
UnknownExpression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Not available
S-EPMC4430420 | biostudies-literature
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