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TranscriptomicsTranscriptional profiling of the developmental gene whiA deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.
WhiA is an unusual transcriptional regulator found in Streptomyces species related to eukaryotic homing endonucleases. It plays a central role in sporulation in these bacteria. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the wh...
ORGANISM(S): Streptomyces venezuelae 
2020-12-11 | E-MTAB-9728 | biostudies-arrayexpress
ProteomicsMycoplasma gallisepticum WhiA knockdown and overexpression
Proteomes of Mycoplasma gallisepticum strains with overexpression and knockdown of WhiA transcription factor. The overexpression was introduced on a transposon vector carrying M. gallisepticum whiA gene with strong constitutive promoter and strong SD sequence. The knockdown was made by CRISPRi. dCas...
ORGANISM(S): Mycoplasma gallisepticum S6 
2021-06-25 | PXD026928 | Pride
Whia Transcription factor Knockdown Overexpression Mycoplasma 
TranscriptomicsWhiA and WhiB of Streptomyces venezuelae depend on each other for binding DNA
WhiB regulon, as determined by ChIP-Seq turned out to be identical to the WhiA regulon leading to the speculation that the two proteins might be required to work together to bind DNA. In this experiment WhiA ChIP-Seq was done in a whiB deletion background and, WhiB ChIP-Seq was done in a whiA deleti...
ORGANISM(S): Streptomyces venezuelae 
2021-07-01 | E-MTAB-10673 | biostudies-arrayexpress
TranscriptomicsMutation of the cysteine residues that coordinate the WhiB [4Fe-4S] cluster prevents in vivo DNA binding by both WhiB and WhiA
The purpose of this experiment was to show that the iron-sulphur cluster ([4Fe-4S]) of whiB is essential for the DNA binding by WhiB and WhiA. The \\"serine version of whiB\\" mentioned below is a whiB in which the 4 cysteine residues which coordinate the [4Fe-4S] cluster were mutated to serines. Ch...
ORGANISM(S): Streptomyces venezuelae 
2021-08-16 | E-MTAB-10818 | biostudies-arrayexpress
GenomicsGenes required for aerial growth, cell division, and chromosome segregation are targets of WhiA before sporulation in Streptomyces venezuelae.
WhiA is a highly unusual transcriptional regulator related to a family of eukaryotic homing endonucleases. WhiA is required for sporulation in the filamentous bacterium Streptomyces, but WhiA homologues of unknown function are also found throughout the Gram-positive bacteria. To better understand th...
ORGANISM(S): Streptomyces venezuelae 
2016-05-20 | E-MTAB-4673 | biostudies-arrayexpress
GenomicsChIP-seq of N-terminal FLAG tagged WhiA
ChIP-seq of N-terminal FLAG tagged WhiA
PRJEB103860 | ENA
GenomicsIDAP-seq of recombinant WhiA incubated with mycobacterium tuberculosis genomic DNA
In vitro DNA affinity purification sequencing of recombinant WhiA incubated with sheard genomic DNA.
ORGANISM(S): Mycobacterium tuberculosis 
2026-08-06 | E-MTAB-16129 | biostudies-arrayexpress
GenomicsChIP-seq of C-terminal FLAG-tagged WhiA
ChIP-seq of C-terminal FLAG-tagged WhiA
PRJEB104001 | ENA
TranscriptomicsThe conserved WhiA protein influences the fatty acid composition of the Bacillus subtilis cell membrane
The conserved WhiA protein family is present in most Gram-positive bacteria and plays a role in cell division. WhiA contains a DNA-binding motive and has been identified as a transcription factor in the actinomycetes. In Bacillus subtilis, a deletion of whiA influences cell division and chromosome s...
ORGANISM(S): Bacillus subtilis 
2019-10-18 | GSE121479 | GEO
GenomicsIDAP-seq of recombinant WhiA incubated with mycobacterium tuberculosis genomic DNA
IDAP-seq of recombinant WhiA incubated with mycobacterium tuberculosis genomic DNA
PRJEB103097 | ENA
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